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Volume 71, Issue 12

sp. nov., a marine bacterium isolated from sediment of South China Sea, and emended descriptions of , and No Access

Abstract

A Gram-stain-negative, motile, facultative anaerobic and rod-shaped bacterium, designated strain NR704-98, was isolated from marine sediment of the northern South China Sea. Cells were positive for oxidase and catalase activity. Growth was observed at 4–30 °C (optimum 20–25 °C), at pH 6–9 (pH 7) and with 0.5–7 % NaCl (2 %). The 16S rRNA gene-based phylogenetic analysis revealed that the nearest phylogenetic neighbours of strain NR704-98 were MS32 (97.9 %), 281 (97.1 %), HAW-EB3 (96.8 %) and HAW-EB2 (96.7 %). Based on the results of phylogenomic analysis, the average nucleotide identity and the digital DNA–DNA hybridization values between strain NR704-98 and the previously mentioned type strains of species of the genus were in the range of 74.9–93.1 % and 20.6–51.4 %, respectively. The respiratory quinones were Q-7 and Q-8. The predominant fatty acids (>10 %) of strain NR704-98 were C, summed feature 3 (C 7 and/or C 6) and iso-C. Phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminophospholipids and five unidentified lipids were detected in strain NR704-98. Based on the phylogenetic and phenotypic characteristics, strain NR704-98 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NR704-98 (=KCTC 82799=MCCC 1K06091).

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Keyword(s): Gammaproteobacteria , polyphasic taxonomy , Shewanella nanhaiensis and Shewanellaceae
Funding
This study was supported by the:
  • the State Key Laboratory of Microbial Technology Open Projects Fund (Award M2020-03)
    • Principle Award Recipient: Wen-RuiCao
  • Fundamental Research Funds of Shandong University (Award 2019HW022)
    • Principle Award Recipient: Ying-JieLi
  • National Natural Science Foundation of China (Award 41976202)
    • Principle Award Recipient: Ming-YuJiang
  • the Strategic Priority Research Program of the Chinese Academy of Sciences (Award XDB42000000)
    • Principle Award Recipient: NotApplicable
  • Open Fund of the Key Laboratory of Marine Geology and Environment, Chinese Academy of Sciences (Award MGE2020KG14)
    • Principle Award Recipient: Wen-RuiCao
  • Natural Science Foundation of Shandong Province (Award ZR2020MD088)
    • Principle Award Recipient: Wen-RuiCao
© 2021 The Authors
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Shewanella nanhaiensis sp. nov., a marine bacterium isolated from sediment of South China Sea, and emended descriptions of Shewanella woodyi, Shewanella hanedai and Shewanella canadensis
Int J Syst Evol Microbiol 71, 005152 (2021); https://doi.org/10.1099/ijsem.0.005152
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