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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 12

gen. nov., sp. nov., a novel anaerobic marine mollicute, and proposal of three novel genera to reclassify members of clusters II–IV No Access

Abstract

A novel strictly anaerobic chemoorganotrophic bacterium, designated Mahy22, was isolated from sulfidic bottom water of a shallow brackish meromictic lake in Japan. Cells of the strain were Gram-stain-negative, non-motile and coccoid in shape with diameters of about 600–800 nm. The temperature range for growth was 15–37 °C, with optimum growth at 30–32 °C. The pH range for growth was pH 6.2–8.9, with optimum growth at pH 7.2–7.4. The strain grew with NaCl concentrations of 5% or below (optimum, 2–3%). Growth of the strain was enhanced by the addition of thiosulfate. The major cellular fatty acids were C and anteiso-C. Respiratory quinones were not detected. The complete genome sequence of strain Mahy22 possessed a 1 885 846 bp circular chromosome and a 12 782 bp circular genetic element. The G+C content of the genome sequence was 30.1 mol%. Phylogenetic analysis based on the 16S rRNA gene revealed that the novel strain belonged to the family , class . The closest relative of strain Mahy22 with a validly published name was J233 with a 16S rRNA gene sequence similarity of 90.5%. Based on the results of polyphasic analysis, the name gen. nov., sp. nov. is proposed to accommodate strain Mahy22, along with reclassification of some species into gen. nov., gen. nov. and gen. nov.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Acholeplasma , anaerobe , marine free-living mollicutes , Mollicutes and novel genera
© 2021 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005138
2021-12-07
2024-05-07
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Mariniplasma anaerobium gen. nov., sp. nov., a novel anaerobic marine mollicute, and proposal of three novel genera to reclassify members of Acholeplasma clusters II–IV
Int J Syst Evol Microbiol 71, 005138 (2021); https://doi.org/10.1099/ijsem.0.005138
/content/journal/ijsem/10.1099/ijsem.0.005138
/content/journal/ijsem/10.1099/ijsem.0.005138
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