Pseudomonas tohonis sp. nov., isolated from the skin of a patient with burn wounds in Japan

Kageto Yamada; Masakazu Sasaki; Kotaro Aoki; Tatsuya Nagasawa; Hinako Murakami; Mayumi Ishii; Kazutoshi Shibuya; Toshisuke Morita; Yoshikazu Ishii; Kazuhiro Tateda

doi:10.1099/ijsem.0.005115

Volume 71, Issue 11

Research Article

Pseudomonas tohonis sp. nov., isolated from the skin of a patient with burn wounds in Japan

Kageto Yamada^1,2, Masakazu Sasaki^1,2, Kotaro Aoki², Tatsuya Nagasawa³, Hinako Murakami¹, Mayumi Ishii⁴, Kazutoshi Shibuya⁴, Toshisuke Morita⁵, Yoshikazu Ishii² and Kazuhiro Tateda²
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Affiliations: ¹ Department of Clinical Laboratory, Toho University Omori Medical Center, 6-11-1 Omori-nishi, Ota-ku, Tokyo 143-8541, Japan ² Department of Microbiology and Infectious Disease, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, Tokyo 143-8540, Japan ³ Department of Clinical Laboratory, Juntendo University Hospital, 3-1-3, Hongo, Bunkyo-ku, Tokyo 113-8431, Japan ⁴ Department of Pathology, Toho University Omori Medical Center, 6-11-1, Omori-nishi, Ota-ku, Tokyo, 143-8541, Japan ⁵ Department of Laboratory Medicine, Toho University School of Medicine, 5-21-6 Omori-nishi, Ota-ku, Tokyo 143-8540, Japan
*Correspondence: Kageto Yamada, [email protected]
Published: 11 November 2021 https://doi.org/10.1099/ijsem.0.005115

Abstract

Strain TUM18999T was isolated from the skin of a patient with burn wounds in Japan. The strain was successfully cultured at 20–42 °C (optimum, 30–35 °C) in 1.0–4.0% NaCl (w/v) and at pH 5.5–9.5, optimum pH 5.5–8.5. The phylogenetic tree reconstructed using 16S rRNA, gyrB, rpoB and rpoD gene sequences indicated that strain TUM18999T is closely related to Pseudomonas otitidis MCC10330T. Although the partial 16S rRNA gene sequence (1412 bp) of TUM18999T exhibits high similarity to those of Pseudomonas alcaligenes NBRC 14159T (99.08 %) and Pseudomonas otitidis MCC10330T (98.51 %), multi-locus sequence analysis using 16S rRNA, gyrB, rpoB and rpoD genes reveals a clear distinction between TUM18999T and other Pseudomonas species. In addition, an average nucleotide identity >90 % was not observed in the P. aeruginosa group. Moreover, TUM18999T and P. otitidis can be distinguished based on the minimum inhibitory concentration for carbapenem. Meanwhile, the cellular fatty acids are enriched with C18 : 1 ω7c/C18 : 1 ω6c (34.35 %), C16 : 1 ω7c/C16 : 1 ω6c (24.22 %), C16 : 0 (19.79 %) and C12 : 0 (8.25 %). Based on this evidence, strain TUM18999T can be defined as representing a novel Pseudomonas species, with the proposed name Pseudomonas tohonis sp. nov. The type strain is TUM18999T (GTC 22698T=NCTC 14580T).

Received: 29/07/2021
Accepted: 01/10/2021
Published Online: 11/11/2021

Keyword(s): average nucleotide identity , gyrB , multi-locus sequence analysis , Pseudomonas tohonis and subclass B3 metallo-β-lactamase

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Pseudomonas tohonis sp. nov., isolated from the skin of a patient with burn wounds in Japan

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Volume 71, Issue 11

Research Article

Pseudomonas tohonis sp. nov., isolated from the skin of a patient with burn wounds in Japan

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