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Volume 71, Issue 11

sp. nov., isolated from the root of in north-west PR China No Access

Abstract

A bacterial strain, designated YZGR15, was isolated from the root of an annual halophyte , collected from the southern edge of the Gurbantunggut desert, north-west PR China. Cells of the isolate were Gram-stain-positive, facultatively anaerobic, irregular rods. Growth occurred at 4–42 °C (optimum, 30–37 °C), at pH 6.0–9.0 (optimum, pH 7.0–7.5) and in the presence of 0–9 % (w/v) NaCl (optimum, 2–5 %). Phylogenetic analysis using 16S rRNA gene sequences indicated that strain YZGR15 showed the highest sequence similarity to (98.27 %) (98.20 %) and (98.06 %). Results of genome analyses of strain YZGR15 indicated that the genome size was 3.16 Mb, with a genomic DNA G+C content of 71.9 mol%. Average nucleotide identity and digital DNA–DNA hybridization values between strain YZGR15and three type strains were in the range of 76.5–77.8 % and 20.0–22.2 %, respectively. Analysis of the cellular component of strain YZGR15 revealed that the primary fatty acids were anteiso-C, C, C and iso-C and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids and two unidentified glycolipids. The cell-wall characteristic amino acids were glutamic acid, alanine and an unknown amino acid. The whole-cell sugars for the strain were mannose, ribose, rhamnose, glucose and an unidentified sugar. The predominant respiratory quinone was MK-9(H). Based on the results of genomic, phylogenetic, phenotypic and chemotaxonomic analyses, strain YZGR15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YZGR15 (=CGMCC 1.18691=KCTC 49659)

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): facultative anaerobe , genome , Gurbantunggut desert , haloalkaline ecosystem , halophyte , Sanguibacter suaedae and Suaeda aralocaspica
Funding
This study was supported by the:
  • the open subject of the Key Laboratory of the Autonomous Region (Award 02017D0408)
    • Principle Award Recipient: Deng-DiAn
  • Scientific projects of colleges and universities in Xinjiang Autonomous Region (Award XJEDU2018I016)
    • Principle Award Recipient: Deng-DiAn
  • National Natural Science Foundation of China (Award 32061143043)
    • Principle Award Recipient: Wen-JunLi
  • National Natural Science Foundation of China (Award 31570109)
    • Principle Award Recipient: Deng-DiAn
© 2021 Sun Yat-Sen University
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2024-04-29
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Sanguibacter suaedae sp. nov., isolated from the root of Suaeda aralocaspica in north-west PR China
Int J Syst Evol Microbiol 71, 005108 (2021); https://doi.org/10.1099/ijsem.0.005108
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