1887

Abstract

A novel symbiotic bacterium, designated strain XY-114, was isolated from the cerata of an marine invertebrate species collected in the South China Sea. Strain XY-114 was an aerobic, Gram-stain-negative, non-motile and short rod-shaped bacterium (0.5–0.8 µm wide and 1.0–1.5 µm long) without flagellum. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XY-114 belonged to the genus with the highest similarity of 97.2 % to the closest phylogenetic relative KYW371. Cells grew at 15–37 °C (optimum, 30 °C), at pH 5.5–9.0 (optimum 7.0–8.0) and at NaCl concentrations of 0.5–5.0 % (w/v; optimum 1.5–3.0 %). The major fatty acids (>10 %) were summed feature 3 (comprising C 7 and/or C 6), iso-C, iso-C G and iso-C 3-OH. The predominant polar lipid was phosphatidylethanolamine. The predominant respiratory quinone was MK-6. Flexirubin-type pigments were absent. The genome size of strain XY-114 was 3.4 Mbp, with 34.9 mol% of DNA G+C content. The average nucleotide identity, digital DNA–DNA hybridization and amino acid identity values between strain XY-114 and KYW371 were 74.5 %, 17.0±1.8 % and 73.9 %. Characterization based on phylogenetic, phenotypic, chemotaxonomic and genomic evidence demonstrated that strain XY-114 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is XY-114 (=KCTC 72217=MCCC 1K03606).

Funding
This study was supported by the:
  • research and development foundation of science and technology of shenzhen grant (Award JCYJ20180305123932369)
    • Principle Award Recipient: YingXu
  • national key r&d program of china (Award 2018YFA0902500)
    • Principle Award Recipient: YuZhang
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2021-11-17
2022-12-08
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