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Volume 71, Issue 11

sp. nov., isolated from hyperthermophilic compost No Access

Abstract

Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423 and FJAT-54424, were isolated from hyperthermophilic compost sampled in Shanxi Province, PR China. Growth was observed at 30–60 °C (optimum, 50 °C) and pH 6.0–9.0 (optimum, pH 7.0), with up to 2.0 % (w/v) NaCl (optimum, 0 % NaCl). The 16S rRNA gene sequence similarity between FJAT-54423 and FJAT-54424 was 99.9%, and the maximum similarity to a valid taxon was observed with (98.3%). Further, in phylogenetic and phylogenomic trees, strains FJAT-54423 and FJAT-54424 branched with members of the genus . The menaquinone was MK-7, and the major fatty acids were iso-C and anteiso-C. The main polar lipids included phosphatidylmethylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The cell-wall peptidoglycan was found to contain -diaminopimelic acid. The DNA G+C content of strains FJAT-54423 and FJAT-54424 were 54.3 and 54.4 mol%, respectively. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values of strain FJAT-54423 and its most closely related reference strain DSM 6347 were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%). Based on the observed physiological properties, chemotaxonomic characteristics and ANI and dDDH values, FJAT-54423 and FJAT-54424 belong to a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FJAT-54423 (=GDMCC 1.2054=KCTC 43273).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Brevibacillus composti sp. nov. , hyperthermophilic compost and thermophile
Funding
This study was supported by the:
  • fujian special fund for public interest research (Award 2019R1034-2)
    • Principle Award Recipient: Guo-HongLiu
  • fujian academy of agricultural sciences (Award AGP2018-3)
    • Principle Award Recipient: Guo-HongLiu
© 2021 The Authors
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2021-11-11
2024-05-02
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Brevibacillus composti sp. nov., isolated from hyperthermophilic compost
Int J Syst Evol Microbiol 71, 005098 (2021); https://doi.org/10.1099/ijsem.0.005098
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