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Volume 71, Issue 11

sp. nov., isolated from tidal mudflat No Access

Abstract

A novel bacterium, designated strain CAU 1637, was isolated from a tidal mudflat. Cells of strain CAU 1637 were Gram-stain-negative, aerobic, motile with single flagellum and rod-shaped. The optimum conditions for growth were observed at 30 °C, pH 6.0 and in the presence of 2 % (w/v) NaCl. The respiratory quinone was ubiquinone-10. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1637 was closely related to the genus , with the highest similarity to NRBC 112946 (97.4 %), followed by NRBC 16783 (96.8 %), JCM 19310 (96.4 %), KCTC 52373 (95.8 %) and JCM 10543 (95.3 %). The predominant cellular fatty acids were C 7 11-methyl and summed feature 8 (C 7 and/or C 6). The major polar lipids consisted of diphosphatidylglycerol and phosphatidylglycerol. The average nucleotide identity values between the novel isolate and related strains ranged from 71.0 to 76.4 %, and the DNA−DNA hybridization values ranged from 19.3 to 20.3 %. The G+C content was 58.4 mol% and the whole-genome size was 4.6 Mb, which included 17 contigs and 3931 protein-coding genes. Based on the taxonomic data, strain CAU 1637 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CAU 1637 (=KCTC 82429=MCCC 1K06080).

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  • Accepted:
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Keyword(s): 16S rRNA gene sequence , Alphaproteobacteria , genome , Rhodobacteraceae , Roseibium limicola and tidal mudflat
Funding
This study was supported by the:
  • Chung-Ang University (Award 2020)
    • Principle Award Recipient: VeerayaWeerawongwiwat
  • National Institute of Biological Resources (Award NIBR202002203)
    • Principle Award Recipient: WonyongKim
© 2021 The Authors
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2021-11-30
2024-05-06
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Roseibium limicola sp. nov., isolated from tidal mudflat
Int J Syst Evol Microbiol 71, 005097 (2021); https://doi.org/10.1099/ijsem.0.005097
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