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Volume 71, Issue 11

sp. nov. and sp. nov., isolated from soil No Access

Abstract

Two Gram-staining-negative, aerobic, rod-shaped bacteria designated strains SR9 and UL070, were isolated from soil and subjected to taxonomic characterization. Strain SR9 grew at 10–37 °C (optimum 30 °C), at pH 4.0–10.0 (optimum pH 8.0) and in the presence of 0–1 % NaCl (optimum 0 %), and UL070 at 4–33 °C (optimum 30 °C), at pH 4.0–10.0 (optimum pH 7.0) and in the presence of 0–2 % NaCl (optimum 0 %), respectively. Strain UL070 was motile with flagella. Analysis of 16S rRNA gene sequences indicated that the two strains fell into phylogenetic clusters belonging to the genus . Both strains SR9 and UL070 were mostly related to S1-A32-2 with 99.70 and 99.01% sequence similarities, and the similarity between the two isolates was 98.90 %. The genome-based analyses indicated that each of the strains SR9 and UL070 was clearly separated from other species of , as the orthologous average nucleotide identity (OrthoANI) and the digital DNA–DNA hybridization (dDDH) values were no higher than 93.09 and 50.03% respectively with any related species, which were clearly below the cutoff for species distinction. The fatty acid profiles of the two strains mainly consisting of unsaturated components, the presence of ubiquinone 9 (Q-9) as the major respiratory quinone, and phosphatidylethanolamine (PE) and diphosphatidylglycerol (DPG) as the diagnostic polar lipids were consistent with their classification into . The DNA G+C contents of strains SR9 and UL070 were 63.2 mol% and 63.6 mol% respectively. On the basis of both phenotypic and phylogenetic evidences, each of the isolated strains should be classified as a novel species, for which the names sp. nov. (type strain=SR9=KCTC 82228=JCM 34509) and sp. nov. (type strain=UL070=KCTC 82229=JCM 34510) are proposed.

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Keyword(s): biosynthetic gene cluster , Pseudomonas guryensis , Pseudomonas ullengensis and soil
Funding
This study was supported by the:
  • National Institute of Biological Resources
© 2021 The Authors
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2021-11-12
2024-05-02
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Pseudomonas guryensis sp. nov. and Pseudomonas ullengensis sp. nov., isolated from soil
Int J Syst Evol Microbiol 71, 005082 (2021); https://doi.org/10.1099/ijsem.0.005082
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