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Volume 71, Issue 11

gen. nov., sp. nov., a new member of the family isolated from the gut of a superworm No Access

Abstract

A novel bacterial strain, designated IPMB12, isolated from the gut of the superworm in Taiwan, was characterized using a polyphasic taxonomic approach. Cells were Gram-stain-negative, facultatively anaerobic, non-motile, coccoid or rod-shaped and formed translucent colonies. Optimal growth occurred at 25–37 °C, pH 9–10, and with 0–2 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain IPMB12 is affiliated with genus in the the family in the class . Strain IPMB12 was most closely related to LMG 29880 with a 94.6 % 16S rRNA gene sequence similarity. Strain IPMB12 showed less than 71.6 % average nucleotide identity, less than 71.5 % average amino acid identity and less than 21.2 % digital DNA–DNA hybridization identity compared to the strains of related genera within the family . The major fatty acids of strain IPMB12 were summed feature 8 (C18 : 17 and/or C18 : 16), C and C. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one uncharacterized phosphoaminoglycolipid and one uncharacterized aminophospholipid. The major isoprenoid quinone was Q-8. Genomic DNA G+C content of strain IPMB12 was 39.3 mol%. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain IPMB12 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is IPMB12 (=BCRC 80908 =LMG 32079=KCTC 82347=KACC 22323).

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  • Accepted:
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Keyword(s): Gammaproteobacteria , Orbaceae , Orbales , Zophobas morio and Zophobihabitans entericusgen. nov., sp. nov
© 2021 The Authors
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2021-11-08
2024-05-02
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Zophobihabitans entericus gen. nov., sp. nov., a new member of the family Orbaceae isolated from the gut of a superworm Zophobas morio
Int J Syst Evol Microbiol 71, 005081 (2021); https://doi.org/10.1099/ijsem.0.005081
/content/journal/ijsem/10.1099/ijsem.0.005081
/content/journal/ijsem/10.1099/ijsem.0.005081
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