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Abstract

A novel marine bacterium, designated strain CHFG3-1-5, was isolated from mangrove sediment sampled at Jiulong River estuary, Fujian, PR China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CHFG3-1-5 belonged to the genus , with the highest sequence similarity to SS011B1-4 (97.6%), followed by D15-8W (97.5%), T17 (97.1%) and SP.17 (90.6%). The bacterium was Gram-stain-negative, facultative anaerobic, oxidase- and catalase-positive, rod-shaped and motile with a polar flagellum. Strain CHFG3-1-5 grew optimally at 32–37 °C, pH 6.0–8.0 and in the presence of 2.0–3.0% (w/v) NaCl. The G+C content of the chromosomal DNA was 61.1 mol%. The major respiratory quinone was determined to be Q-9. The principal fatty acids were C, summed feature 3 (C 7/6), C, summed feature 9 (C iso 9 and/or C 10-methyl), C 3-OH and summed feature 8 (C 7 and/or C 6). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three phospholipids, one glycolipid and two aminolipids. The average nucleotide identity and digital DNA–DNA hybridization values among the genomes of strain CHFG3-1-5 and the reference strains were 73.4–79.4 and 19.6–22.4%, respectively. Like many other species reported in the genus , strain CHFG3-1-5 was able to oxidise iron. The combined genotypic and phenotypic data showed that strain CHFG3-1-5 represents a novel species within the genus , for which the name sp. nov. is proposed, with the type strain CHFG3-1-5 (=MCCC 1A18306=KCTC 82398).

Funding
This study was supported by the:
  • the fund of national microbial resource center (Award No. NMRC-2020-9)
    • Principle Award Recipient: ZongzeShao
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2021-11-11
2024-07-25
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