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Volume 71, Issue 9

sp. nov., a novel actinomycete isolated from the rhizosphere of No Access

Abstract

An aerobic, Gram-stain-positive, non-motile, dull-yellow, short rod-shaped actinomycete strain, designated G10, was isolated from (marigold) roots collected from Goyang in the Republic of Korea. The isolate showed best growth on Reasoner's 2A agar at 25 °C, pH 6.5.0 and with 0% NaCl (w/v). The strain was negative for oxidase activity and positive for catalase activity. On the basis of 16S RNA gene sequence similarity, strain G10 was affiliated to the genus and the closest species were HLT3-15 (98.8 %), HLT2-9 (98.8 %), JC2055 (98.7 %), YIM A1136 (98.6 %), Y4 (98.5 %), DSM 16090 (98.3 %), Cr7-14 (98.2 %), DSM 22017 (98.1 %) and KCTC 19197 (98.1 %). Strain G10 formed a monophyletic cluster with HLT3-15, HLT2-9 and KCTC 19197 in all phylogenetic trees. The cell-wall peptidoglycan of strain G10 contained -diaminopimelic acid as the diagnostic amino acid. The predominant fatty acids were iso-C and C 8. MK-8(H) was the major isoprenoid quinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. Average nucleotide identity and digital DNA–DNA hybridization values were 78.6–88.7 % and 21.5–36.2 %, respectively, with the type strains of related species of the genus , suggesting that strain G10 represents a novel species. The genome of strain G10 is 4 231 000 bp long with a DNA G+C content of 71.5 mol% and encodes 4071 predicted proteins, six rRNAs and 46 tRNAs. The genome of strain G10 comprises the biosynthetic gene cluster for T3PKS, terpene, NRPS-like fragment and RRE-containing element as secondary metabolites. The results of taxonomic, phylogenetic, biochemical, chemotaxonomic and genomic analysis clearly supported that strain G10 represent a novel species within the genus , for which the name sp. nov is proposed and the type strain is G10 (=KCTC 49626=NBRC 114801).

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Keyword(s): Actinomycete , Asteraceae , Nocardiodes baculatus , Nocardioides and Tagetes patula
© 2021 The Authors
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2021-09-20
2024-05-14
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Nocardioides baculatus sp. nov., a novel actinomycete isolated from the rhizosphere of Tagetes patula
Int J Syst Evol Microbiol 71, 005012 (2021); https://doi.org/10.1099/ijsem.0.005012
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