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Abstract

A Gram-stain-positive, non-motile and short rod-shaped actinobacterium, designated strain LNNU 22110, was isolated from the rhizosphere soil of the halophyte (Bunge) Freitag and Schütze, which collected in Xinjiang, north-west China. Growth occurred at 10–45 °C, pH 6.0–10.0 and in the presence of 0–11 % NaCl (w/v). Based on the results of 16S rRNA gene sequence phylogenetic analyses, strain LNNU 22110 belonged to the genus and had 97.5 and 95.5 % sequence similarity to KCTC 19413 and CGMCC 4.3142, respectively. The digital DNA–DNA hybridization relatedness values between strain LNNU 22110 and KCTC 19413 and CGMCC 4.3142 were 23.2 and 19.9 %, respectively. The highest average nucleotide identity value between strain LNNU 22110 and its closest related strain ( KCTC 19413) was 80.2 %, much lower than the species delineation threshold of 95–96 %. The genome of strain LNNU 22110 was 4.4 Mb, with a genomic DNA G+C content of 68.4 mol%. The diagnostic diamino acids in the peptidoglycan layer of strain LNNU 22110 were lysine, alanine, glycine, glutamic acid and aspartic acid. The predominant menaquinone was MK-8(H). The major fatty acid (>10 %) was anteiso-C. The polar lipid profile of strain LNNU 22110 included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, diacylated phosphatidyl dimannoside, one unidentified glycolipid and two unidentified phospholipids. According to the phenotypic, phylogenetic and chemotaxonomic results, strain LNNU 22110 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LNNU 22110 (=KCTC 39807=CGMCC 1.17105).

Funding
This study was supported by the:
  • department of education of liaoning province (Award LJ2020011)
    • Principle Award Recipient: Hong-FeiWang
  • natural science foundation of liaoning province (Award 2019-ZD-0466)
    • Principle Award Recipient: Hong-FeiWang
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2021-09-20
2021-10-24
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