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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 9

Three novel species of the group isolated from clinical samples in Japan No Access

Abstract

Three Gram-positive bacterial strains, BML-BC004, BML-BC017 and BML-BC059, isolated from blood samples from three inpatients in Japan, were identified as members of using matrix-assisted laser desorption ionization time-of-flight MS. The 16S rRNA gene sequences of these three strains were more than 97.1 % similar to 18 type strains belonging to the group. Whole-genome comparisons, using average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH), confirmed that the three strains represented three individual distinct species belonging to the group. A phylogenetic tree showed that BML-BC004, BML-BC017 and BML-BC059 were located close to , and , respectively. Based on these phylogenetic and phenotypic data, including values below the threshold for ANI and dDDH, the three strains should be classified as representing three different novel species of the group: sp. nov., with type strain BML-BC004 (=DSM 111102=JCM 34122), sp. nov., with type strain BML-BC017 (=DSM 111100=JCM 34124) and sp. nov., with type strain BML-BC059 (=DSM 111101=JCM 34125).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacillus cereus group and human pathogen
Funding
This study was supported by the:
  • japan agency for medical research and development (Award 20fk0108061h0303)
    • Principle Award Recipient: TeruoKirikae
  • japan society for the promotion of science (Award 19K16652)
    • Principle Award Recipient: MariTohya
© 2021 The Authors
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2021-09-08
2024-04-28
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Three novel species of the Bacillus cereus group isolated from clinical samples in Japan
Int J Syst Evol Microbiol 71, 004993 (2021); https://doi.org/10.1099/ijsem.0.004993
/content/journal/ijsem/10.1099/ijsem.0.004993
/content/journal/ijsem/10.1099/ijsem.0.004993
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