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Volume 71, Issue 8

sp. nov., isolated from marine sponge Free

Abstract

A novel bacterial strain, designated as HN-E44, was isolated from marine sponge collected from Yangpu Bay, Hainan, PR China. Strain HN-E44 was Gram-stain-negative, non-motile, catalase-positive, oxidase-negative, rod-shaped and yellow-pigmented. Growth occurred at 4–37 °C (optimum, 28 °C), at pH 6–8 (pH 7) and in 0.5–14 % (w/v) NaCl (3–5 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HN-E44 formed an independent cluster with JCM 14121 within the family and had the highest sequence similarity of 93.6 % to the closest type strain JCM 14121. The major fatty acids were iso-C, anteiso-C, iso-C 3-OH, summed feature 3 (C 7 and/or C 6) and iso-C G. The polar lipids comprised phosphatidylethanolamine, sphingolipid, four unidentified phospholipids, an unidentified aminophospholipid and an unidentified lipid. The respiratory quinone was identified as MK-6. The genomic DNA G+C content was determined to be 40.6 mol%. The average nucleotide identity (ANI) and average amino acid identity (AAI) values between strain HN-E44 and closest type strain JCM 14121 were, respectively, 79.6 and 85.2 %, both of which were below thresholds for species delineation (95–96 % ANI and 95–96 % AAI), but were over thresholds for genus delineation (73.98 % ANI and 70–76 % AAI). The combined genotypic and phenotypic distinctiveness demonstrated that strain HN-E44 could be differentiated from closely related genera. Therefore, it is proposed that strain HN-E44 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain HN-E44 (=MCCC 1K03332=LMG 30459).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Marixanthomonas spongiae sp. nov. , polyphasic taxonomy and sponge
Funding
This study was supported by the:
  • Engineering Research Center of Natural Cosmeceuticals College of Fujian Province Fund (Xiamen Medical College) (Award XMMC-NC202004)
    • Principle Award Recipient: ZhuangLingping
  • Young and the Middle-Aged Teacher Education Scientific Research Project of Fujian Province, China (Award JAT200736)
    • Principle Award Recipient: ZhuangLingping
© 2021 The Authors
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2024-12-08
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Marixanthomonas spongiae sp. nov., isolated from marine sponge
Int J Syst Evol Microbiol 71, 004968 (2021); https://doi.org/10.1099/ijsem.0.004968
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