1887

Abstract

An anaerobic, alkaliphilic, halotolerant, Gram-stain-positive and rod-shaped bacterium, designated Q10-2, was isolated from mangrove sediment sampled at the Jiulong river estuary, PR China. The cells of strain Q10-2 were motile and 0.5×2–4 µm in size. Strain Q10-2 grew at 8–45 °C (optimum, 32 °C), at pH 7.0–10.5 (optimum, pH 8.5) and in the presence of 0–6 % (w/v) NaCl (optimum, 3 %). It could use complex organic compounds and carbohydrates including -fructose, -galactose, -glucose, -mannitol, -xylose, trehalose, lactose, maltose, sucrose and starch as carbon sources and electron donors. It could reduce sulphate, thiosulphate and elemental sulphur to sulphide, but not sulphite. Fe (Ⅲ) citrate, ferrihydrite, haematite and goethite in the presence of glucose as the electron donor were also reduced. Acetate, butyrate, ethanol, CO and H were end products of glucose fermentation. The predominant cellular fatty acids were composed of C, C and summed features containing C ω7 and/or iso-C 2-OH and iso-C and/or anteiso-C B. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain was most closely related to DSM 12116 (95.5 % sequence similarity). The genome size of strain Q10-2 was 5.0 Mb, with a G+C content of 37.4 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain Q10-2 and DSM 12116 were 69.1 and 21.8 %, respectively. The combined genotypic and phenotypic data showed that strain Q10-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Q10-2 (=MCCC 1A16257=KCTC 15906).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004952
2021-11-05
2022-12-08
Loading full text...

Full text loading...

References

  1. Ravot G, Magot M, Fardeau M-L, Patel BKC, Thomas P et al. Fusibacter paucivorans gen. nov., sp. nov., an anaerobic, thiosulfate-reducing bacterium from an oil-producing well. Int J Syst Evol Microbiol 1999; 49:1141–1147 [View Article]
    [Google Scholar]
  2. Ben Hania W, Fraj B, Postec A, Fadhlaoui K, Hamdi M et al. Fusibacter tunisiensis sp. nov., isolated from an anaerobic reactor used to treat olive-mill wastewater. Int J Syst Evol Microbiol 2012; 62:1365–1368 [View Article] [PubMed]
    [Google Scholar]
  3. Smii L, Ben Hania W, Cayol JL, Joseph M, Hamdi M et al. Fusibacter bizertensis sp. nov., isolated from a corroded kerosene storage tank. Int J Syst Evol Microbiol 2015; 65:117–121 [View Article] [PubMed]
    [Google Scholar]
  4. Fadhlaoui K, Ben Hania W, Postec A, Fauque G, Hamdi M et al. Fusibacter fontis sp. nov., a sulfur-reducing, anaerobic bacterium isolated from a mesothermic Tunisian spring. Int J Syst Evol Microbiol 2015; 65:3501–3506 [View Article] [PubMed]
    [Google Scholar]
  5. Ruley JA, Tumuhairwe JB, Amoding A, Westengen OT, Vinje H. Rhizobacteria communities of phytoremediation plant species in petroleum hydrocarbon contaminated soil of the Sudd ecosystem, South Sudan. Int J Microbiol 2020; 2:1–13 [View Article]
    [Google Scholar]
  6. Wang Y, Zhang G, Wang H, Cheng Y, Liu H et al. Effects of different dissolved organic matter on microbial communities and arsenic mobilization in aquifers. J Hazard Mater 2021; 411:125146 [View Article] [PubMed]
    [Google Scholar]
  7. Wang J, She J, Zhou Y, Tsang DCW, Beiyuan J et al. Microbial insights into the biogeochemical features of thallium occurrence: A case study from polluted river sediments. Sci Total Environ 2020; 739:139957 [View Article] [PubMed]
    [Google Scholar]
  8. Lagkouvardos I, Joseph D, Kapfhammer M, Giritli S, Horn M et al. IMNGS: A comprehensive open resource of processed 16S rRNA microbial profiles for ecology and diversity studies. Sci Rep 2016; 6:33721 [View Article] [PubMed]
    [Google Scholar]
  9. Hungate RE, Macy Jjm. The roll-tube method for cultivation of strict anaerobes. Appl Environ Microbiol 1973; 3:123–126
    [Google Scholar]
  10. Widdel F, Pfennig N. Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids II. Incomplete oxidation of propionate by Desulfobulbus propionicus gen. nov., sp. nov. Arch Microbiol 1982; 131:360–365 [View Article]
    [Google Scholar]
  11. Lovley DR, Phillips EJP. Organic-matter mineralization with reduction of ferric iron in anaerobic sediments. Appl Environ Microbiol 1986; 51:683–689 [View Article] [PubMed]
    [Google Scholar]
  12. Halebian S, Harris B, Finegold SM, Rolfe RD. Rapid method that aids in distinguishing Gram-positive from Gram-negative anaerobic bacteria. Comparative Study 1981; 13:444–448
    [Google Scholar]
  13. Kang YS, Risbud S, Rabolt JF. Synthesis and characterization of nanometer-size Fe3O4 and Fe2O3 particles. Chem Mater 1996; 8:2209–2211 [View Article]
    [Google Scholar]
  14. Cord-Ruwisch R. A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. J Microbiol Methods 1985; 4:33–36 [View Article]
    [Google Scholar]
  15. Stookey L. Ferrozine; a new spectrophometric reagent for iron. Analytical Chemistry 1970; 42:
    [Google Scholar]
  16. Fardeau ML, Ollivier B, Patel BKC, Magot M, Thomas P et al. Thermotoga hypogea sp. nov., a xylanolytic, thermophilic bacterium from an oil-producing well. Int J Syst Evol Microbiol 1997; 47:1013–1019
    [Google Scholar]
  17. Miller LT. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 1982; 16:584–586 [View Article] [PubMed]
    [Google Scholar]
  18. Kuykendall LD, Roy MA, O'Neill JJ, Devine TE. Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Evol Microbiol 1988; 38:358–361
    [Google Scholar]
  19. Wilson KH, Blitchington RB, Greene RC. Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction. J Clin Microbiol 1990; 28:1942–1946 [View Article] [PubMed]
    [Google Scholar]
  20. Chun J, Lee JH, Jung Y, Kim M, Kim S et al. EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 2007; 57:2259–2261 [View Article] [PubMed]
    [Google Scholar]
  21. Scott MG, Madden T. blast: at the core of a powerful and diverse set of sequence analysis tools. Nucleic Acids Res 2004; suppl_2:W20
    [Google Scholar]
  22. Thompson JD, Higgins DG, Gibson TJ. clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22:4673–4680 [View Article] [PubMed]
    [Google Scholar]
  23. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article] [PubMed]
    [Google Scholar]
  24. Overbeek R, Olson R, Pusch GD, Olsen GJ, Stevens R. The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST. Nucleic Acids Res 2013; 42:D206–14 [View Article] [PubMed]
    [Google Scholar]
  25. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012; 19:455–477 [View Article] [PubMed]
    [Google Scholar]
  26. Na S-I, Kim YO, Yoon S-H, Ha S-M, Baek I et al. UBCG: Up-to-date bacterial core gene set and pipeline for phylogenomic tree reconstruction. J Microbiol 2018; 56:280–285 [View Article] [PubMed]
    [Google Scholar]
  27. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014; 30:1312–1313 [View Article] [PubMed]
    [Google Scholar]
  28. Yoon S-H, Ha S-M, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017; 110:1281–1286 [View Article] [PubMed]
    [Google Scholar]
  29. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article] [PubMed]
    [Google Scholar]
  30. Garber AI, Nealson KH, Okamoto A, McAllister SM, Chan CS et al. FeGenie: a comprehensive tool for the identification of iron genes and iron gene neighborhoods in genome and metagenome assemblies. Front Microbiol 2020; 11:37 [View Article] [PubMed]
    [Google Scholar]
  31. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article] [PubMed]
    [Google Scholar]
  32. Rodriguez-R LM, Konstantinidis KT. The enveomics collection: a toolbox for specialized analyses of microbial genomes and metagenomes. Peer J Preprints Preprint [View Article]
    [Google Scholar]
  33. Shirodkar S, Reed S, Romine M, Saffarini D. The octahaem SirA catalyses dissimilatory sulfite reduction in Shewanella oneidensis MR-1. Environ Microbiol 2011; 13:108–115 [View Article] [PubMed]
    [Google Scholar]
  34. Burns JL, DiChristina TJ. Anaerobic respiration of elemental sulfur and thiosulfate by Shewanella oneidensis MR-1 requires psrA, a homolog of the phsA gene of Salmonella enterica serovar typhimurium LT2. Appl Environ Microbiol 2009; 75:5209–5217 [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004952
Loading
/content/journal/ijsem/10.1099/ijsem.0.004952
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error