1887

Abstract

A thermophilic, anaerobic, iron-reducing bacterium strain JL129W03 (=KCTC 15905=MCCC 1A14213) was isolated from a sulfide sample collected from the Daxi hydrothermal field (60.5° E, 6.4° N, 2919 m depth) on the Carlsberg Ridge, northwest Indian Ocean. Cells grew at 55–75 °C(optimum, 70 °C), at pH 6.0–9.0 (optimum, pH 6.0–7.0) and at NaCl concentrations of 1.5–4.5 % (w/v; optimum 3.0 %). Under optimal growth conditions, the generation time was around 85 min. The isolate was an obligate chemoorganoheterotroph, utilizing complex organic compounds, carbohydrates, organic acids and one amino acid. It was anaerobic and facultatively dependent on elemental sulphur and various forms of Fe(III) as an electron acceptor: insoluble forms and soluble forms. It did not reduce sulfite, sulphate, thiosulfate or nitrate. The G+C content of its genomic DNA was 34.0 mol%. Phylogenetic 16S rRNA gene sequence analyses revealed that its closest relative was DV1140 with 95.81 % 16S rRNA sequence similarity. On the basis of physiological distinctness and phylogenetic distance, the isolate is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain JL129W03 (=KCTC 15905;=MCCC 1A14213).

Funding
This study was supported by the:
  • China Ocean Mineral Resources Research and Development Association (Award DY135-B2-01)
    • Principle Award Recipient: ZongzeShao
  • National Natural Science Foundation of China (Award 91951201)
    • Principle Award Recipient: XiqiuHan
  • Key Technology Research and Development Program of Shandong (Award 2018YFC0310701)
    • Principle Award Recipient: ZongzeShao
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004929
2021-07-30
2024-05-02
Loading full text...

Full text loading...

References

  1. Vargas M, Kashefi K, Blunt-Harris EL, Lovley DR. Microbiological evidence for Fe(III) reduction on early earth. Nature 1998; 395:65–67 [View Article]
    [Google Scholar]
  2. Zeng X, Zhang Z, Li X, Jebbar M, Alain K et al. Caloranaerobacter ferrireducens sp. nov., an anaerobic, thermophilic, iron (III)-reducing bacterium isolated from deep-sea hydrothermal sulfide deposits. Int J Syst Evol Microbiol 2015; 65:1714–1718 [View Article] [PubMed]
    [Google Scholar]
  3. Kendall MM. Isolation of a Novel Thermophilic, Iron Reducing Bacterium from a Deep-Sea Hydrothermal Vent USA: Portland State University; 2002
    [Google Scholar]
  4. Antoine E, Cilia V, Meunier JR, Guezennec J, Lesongeur F et al. Thermosipho melanesiensis sp. nov., a new thermophilic anaerobic bacterium belonging to the order Thermotogales, isolated from deep-sea hydrothermal vents in the southwestern Pacific Ocean. Int J Syst Bacteriol 1997; 47:1118–1123 [View Article] [PubMed]
    [Google Scholar]
  5. Kuwabara T, Kawasaki A, Uda I, Sugai A. Thermosipho globiformans sp. nov., an anaerobic thermophilic bacterium that transforms into multicellular spheroids with a defect in peptidoglycan formation. Int J Syst Evol Microbiol 2011; 61:1622–1627 [View Article] [PubMed]
    [Google Scholar]
  6. Podosokorskaya OA, Bonch-Osmolovskaya EA, Godfroy A, Gavrilov SN, Beskorovaynaya DA et al. Thermosipho activus sp. nov., a novel thermophilic anaerobic hydrolytic bacterium isolated from a deep-sea sample Guaymas Basin, Gulf of California. Int J Syst Evol Microbiol 2014; 64:3307–3313 [View Article] [PubMed]
    [Google Scholar]
  7. Balk M, Weijma J, Stams AJM. Thermotoga lettingae sp. nov., a novel thermophilic, methanol-degrading bacterium isolated from a thermophilic anaerobic reactor. Int J Syst Evol Microbiol 2002; 52:1361–1368 [View Article] [PubMed]
    [Google Scholar]
  8. Slobodkin AI, Jeanthon C, Lharidon S, Nazina TN, Miroshnichenko ML et al. Dissimilatory reduction of Fe(III) by thermophilic bacteria and archaea in deep subsurface petroleum reservoirs of western siberia. Curr Microbiol 1999; 39:99–102 [View Article] [PubMed]
    [Google Scholar]
  9. Fardeau ML, Goulhen F, Bruschi M, Khelifi N, Cayol JL et al. Archaeoglobus fulgidus and Thermotoga elfii, thermophilic isolates from deep geothermal water of the Paris Basin. Geomicrobiol J 2009; 26:119–130 [View Article]
    [Google Scholar]
  10. Nelson KE, Clayton RA, Gill SR, Gwinn ML, Dodson RJ et al. Evidence for lateral gene transfer between archaea and bacteria from genome sequence of Thermotoga maritima. Nature 1999; 399:323–329 [View Article] [PubMed]
    [Google Scholar]
  11. Bhandari V, Gupta RS. Molecular signatures for the phylum (class) Thermotogae and a proposal for its division into three orders (Thermotogales, Kosmotogales ord. nov. and Petrotogales ord. nov.) containing four families (Thermotogaceae, Fervidobacteriaceae fam. nov., Kosmotogace fam. nov. and Petrotogaceae fam. nov.) and a new genus Pseudothermotoga gen. nov. with five new combinations. Antonie van Leeuwenhoek 2014; 105:143–168 [View Article] [PubMed]
    [Google Scholar]
  12. Urios L, Cueff-Gauchard V, Pignet P, Postec A, Fardeau ML et al. Thermosipho atlanticus sp. nov., a novel member of the Thermotogales isolated from a Mid-Atlantic Ridge hydrothermal vent. Int J Syst Evol Microbiol 2004; 54:1953–1957 [View Article] [PubMed]
    [Google Scholar]
  13. Haridon SL, Miroshnichenko ML, Hippe H, Fardeau ML, Bonch-Osmolovskaya E et al. Thermosipho geolei sp. nov., a thermophilic bacterium isolated from a continental petroleum reservoir in Western Siberia. Int J Syst Evol Microbiol 2001; 51:1327–1334 [View Article] [PubMed]
    [Google Scholar]
  14. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. mega X: Molecular Evolutionary Genetics Analysis across computing platforms. Mole Biol Evol 2018; 35:1547–1549 [View Article]
    [Google Scholar]
  15. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: A taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article] [PubMed]
    [Google Scholar]
  16. Rodriguez-R LM, Konstantinidis KT. The enveomics collection: a toolbox for specialized analyses of microbial genomes and metagenomes. PeerJ Preprints 2016; 4:e1900v1
    [Google Scholar]
  17. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article] [PubMed]
    [Google Scholar]
  18. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 2009; 106:19126–19131 [View Article] [PubMed]
    [Google Scholar]
  19. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Int J Syst Bacteriol 1987; 37:463–464
    [Google Scholar]
  20. Na SI, Kim YO, Yoon SH, Ha S, Baek I. UBCG: Up-to-date bacterial core gene set and pipeline for phylogenomic tree reconstruction. J Microbiol 2018; 56:280–285 [View Article] [PubMed]
    [Google Scholar]
  21. Lovley DR, Phillips EJP. Availability of ferric iron for microbial reduction in bottom sediments of the freshwater tidal potomac river. Appl Environ Microbiol 1986; 52:751–757 [View Article] [PubMed]
    [Google Scholar]
  22. Alain K, Pignet P, Zbinden M. Caminicella sporogenes gen. nov., sp. nov., a novel thermophilic spore-forming bacterium isolated from an East-Pacific Rise hydrothermal vent. Int J Syst Evol Microbiol 2002; 52:1621–1628 [View Article] [PubMed]
    [Google Scholar]
  23. Cline JD. Spectrophotometric determination of hydrogen sulfide in natural waters. Limnol Oceanogr 1969; 14:454–458 [View Article]
    [Google Scholar]
  24. Sasser M. MIDI Technical Note 101. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids Newark, Del: MIDI, Inc; 1990
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004929
Loading
/content/journal/ijsem/10.1099/ijsem.0.004929
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error