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Abstract

A thermophilic, anaerobic, iron-reducing bacterium strain JL129W03 (=KCTC 15905=MCCC 1A14213) was isolated from a sulfide sample collected from the Daxi hydrothermal field (60.5° E, 6.4° N, 2919 m depth) on the Carlsberg Ridge, northwest Indian Ocean. Cells grew at 55–75 °C(optimum, 70 °C), at pH 6.0–9.0 (optimum, pH 6.0–7.0) and at NaCl concentrations of 1.5–4.5 % (w/v; optimum 3.0 %). Under optimal growth conditions, the generation time was around 85 min. The isolate was an obligate chemoorganoheterotroph, utilizing complex organic compounds, carbohydrates, organic acids and one amino acid. It was anaerobic and facultatively dependent on elemental sulphur and various forms of Fe(III) as an electron acceptor: insoluble forms and soluble forms. It did not reduce sulfite, sulphate, thiosulfate or nitrate. The G+C content of its genomic DNA was 34.0 mol%. Phylogenetic 16S rRNA gene sequence analyses revealed that its closest relative was DV1140 with 95.81 % 16S rRNA sequence similarity. On the basis of physiological distinctness and phylogenetic distance, the isolate is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain JL129W03 (=KCTC 15905;=MCCC 1A14213).

Funding
This study was supported by the:
  • China Ocean Mineral Resources Research and Development Association (Award DY135-B2-01)
    • Principle Award Recipient: ZongzeShao
  • National Natural Science Foundation of China (Award 91951201)
    • Principle Award Recipient: XiqiuHan
  • Key Technology Research and Development Program of Shandong (Award 2018YFC0310701)
    • Principle Award Recipient: ZongzeShao
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2021-07-30
2024-06-14
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