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Volume 71, Issue 8

sp. nov., isolated from the seawater of the South Atlantic Ocean No Access

Abstract

A Gram-negative, non-motile, non-spore-forming, aerobic and short rod-shaped bacterial strain R32, was isolated from seawater of the South Atlantic Ocean. Strain R32 grew at 10–40 °C (optimum 28 °C), at pH 6.0–8.0 (optimum 7.0), and in the presence of 3–8 % NaCl (w/v) (optimum 5 %). Cells were oxidase- and catalase-positive. The 16S rRNA gene sequence of strain R32 shared the highest similarities with (98.3 %), followed by (93.0 %), (92.8 %), (92.5 %) and (92.4 %). The dominant fatty acids were iso-C (32.7 %) and iso-C 3-OH (21.1 %). Menaquinone-6 (MK-6) was detected as the sole respiratory quinone. The polar lipids found were phosphatidylethanolamine, three aminolipids and three unidentified lipids. The DNA G+C content was 35.0 mol%. The ANI value and dDDH value between strain R32 and the and species were 70.5–85.8 % and 18.7–30.5 %, respectively. Based on the results of the polyphasic characterization, strain R32 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R32 (=MCCC 1A09780=KCTC 72004).

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  • Accepted:
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Keyword(s): Flavobacteriaceae , marine bacteria , Mesonia hitae sp. nov , phylogeny , South Atlantic Ocean and taxonomy
Funding
This study was supported by the:
  • Fund for scientific innovation
    • Principle Award Recipient: YingZhou
  • National Natural Science Foundation of China (Award 31900088)
    • Principle Award Recipient: YingZhou
  • COMRA (Award DY135-B2-17)
    • Principle Award Recipient: PeishengYan
© 2021 The Authors
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References

  1. Jooste PJ. The Taxonomy and Significance of Flavobacterium–Cytophaga Strains from Daily Sources, PhD Thesis University of the Orange Free State; 1985
     [Google Scholar]
  2. Bernardet J-F, Nakagawa Y. An introduction to the family Flavobacteriaceae. Dworkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E. eds In The Prokaryotes: A handbook on the biology of bacteria, 3rd. edn Vol 7 New York, NY: Springer; 2006 pp 455–480
     [Google Scholar]
  3. Bernardet J-F, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae, and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [PubMed]
     [Google Scholar]
  4. McBride MJ. The family Flavobacteriaceae. Rosenberg E, DeLong E, Lory S, Stackebrandt E, Thompson F. eds In The Prokaryotes-other Major Lineages of Bacteria and the Archaea Berlin, Heidelberg: Springer Berlin Heidelberg; 2014 pp 643–676
     [Google Scholar]
  5. Alonso C, Warnecke F, Amann R, Pernthaler J. High local and global diversity of Flavobacteria in marine plankton. Environ Microbiol 2007; 9:1253–1266 [View Article] [PubMed]
     [Google Scholar]
  6. Kirchman DL. The ecology of Cytophaga-Flavobacteria in aquatic environments. FEMS Microbiol Ecol 2002; 39:91–100 [View Article] [PubMed]
     [Google Scholar]
  7. O’Sullivan LA, Rinna J, Humphreys G, Weightman AJ, Fry JC. Culturable phylogenetic diversity of the phylum ‘Bacteroidetes’ from river Epilithon and coastal water and description of novel members of the family Flavobacteriaceae: Epilithonimonas tenax gen. nov., sp. nov. and Persicivirga xylanidelens gen. nov., sp. nov. Int J Syst Evol Microbiol 2006; 56:169–180 [View Article] [PubMed]
     [Google Scholar]
  8. Nedashkovskaya OI, Kim SB, Han SK, Lysenko AM, Rohde M et al. Mesonia algae gen. nov.,sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi (Kütz) Kornm. Int J Syst Evol Microbiol 2003; 53:1967–1971 [View Article] [PubMed]
     [Google Scholar]
  9. Nedashkovskaya OI, Kim SB, Zhukova NV, Kwak J, Mikhailov VV. Mesonia mobilis sp. nov., isolated from seawater, and emended description of the genus Mesonia. Int J Syst Evol Microbiol 2006; 56:2433–2436 [View Article] [PubMed]
     [Google Scholar]
  10. Kang HS, Lee SD. Mesonia phycicola sp. nov., isolated from seaweed, and emended description of the genus Mesonia. Int J Syst Evol Microbiol 2010; 60:591–594 [View Article] [PubMed]
     [Google Scholar]
  11. Lee SY, Lee MH, Yoon JH. Mesonia ostreae sp. nov., isolated from seawater of an oyster farm, and emended description of the genus Mesonia. Int J Syst Evol Microbiol 2012; 62:1804–1808 [View Article] [PubMed]
     [Google Scholar]
  12. Choi A, Baek K, Lee H, Cho JC. Mesonia aquimarina sp. nov., a marine bacterium isolated from coastal seawater. Int J Syst Evol Microbiol 2015; 65:135–140 [View Article] [PubMed]
     [Google Scholar]
  13. Kolberg J, Busse HJ, Wilke T, Schubert P, Kampfer P et al. Mesonia hippocampi sp. nov., isolated from the brood pouch of a diseased Barbour’s Seahorse (Hippocampus barbouri. Int J Syst Evol Microbiol 2015; 65:2241–2247 [View Article] [PubMed]
     [Google Scholar]
  14. Wang F-Q, Xie Z-H, Zhao J-X, Chen G-J, Du Z-J. Mesonia sediminis sp. nov., isolated from a sea cucumber culture pond. Antonie Van Leeuwenhoek 2015; 108:1205–1212 [View Article] [PubMed]
     [Google Scholar]
  15. Sung H-R, Joh K, Shin K-S. Mesonia maritima sp. nov., isolated from seawater of the South Sea of Korea. Int J Syst Evol Microbiol 2017; 67:2574–2580 [View Article] [PubMed]
     [Google Scholar]
  16. Lucena T, Sanz-Sáez I, Arahal DR, Acinas SG, Sánchez O et al. Mesonia oceanica sp. nov., isolated from oceans during the Tara oceans expedition, with a preference for mesopelagic waters. Int J Syst Evol Microbiol 2020; 70:4329–4338 [View Article] [PubMed]
     [Google Scholar]
  17. Wang K, Yan P, Ma R, Jia W, Shao Z. Diversity of culturable bacteria in deep-sea water from the South Atlantic Ocean. Bioengineered 2017; 8:572–584 [View Article] [PubMed]
     [Google Scholar]
  18. Aygan A, Arikan B. An overview on bacterial motility detection. Int J Agr Biol 2007; 9:193–196
     [Google Scholar]
  19. Taylor WI, Achanzar D. Catalase test as an aid to the identification of Enterobacteriaceae. Appl Microbiol 1972; 24:58–61 [View Article] [PubMed]
     [Google Scholar]
  20. Tarrand JJ, Gröschel DH. Rapid, modified oxidase test for oxidase-variable bacterial isolates. J Clin Microbiol 1982; 16:772–774 [View Article] [PubMed]
     [Google Scholar]
  21. Lane DJ. 16S/23S rRNA sequencing. Stackebrandt E, Goodfellow M. eds In Nucleic Acid Techniques in Bacterial Systematics John Wiley & Sons; 1991 pp 115–175
     [Google Scholar]
  22. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article] [PubMed]
     [Google Scholar]
  23. Thompson JD, Higgins DG, Gibson TJ. clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22:4673–4680 [View Article] [PubMed]
     [Google Scholar]
  24. Kumar S, Stecher G, Tamura K. mega7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article] [PubMed]
     [Google Scholar]
  25. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article] [PubMed]
     [Google Scholar]
  26. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article] [PubMed]
     [Google Scholar]
  27. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article] [PubMed]
     [Google Scholar]
  28. Rzhetsky A, Nei M. Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. J Mol Evol 1992; 35:367–375 [View Article] [PubMed]
     [Google Scholar]
  29. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012; 19:455–477 [View Article] [PubMed]
     [Google Scholar]
  30. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015; 25:1043–1055 [View Article] [PubMed]
     [Google Scholar]
  31. Overbeek R, Olson R, Pusch GD, Olsen GJ, Davis JJ et al. The SEED and the rapid annotation of microbial genomes using subsystems technology (RAST. Nucleic Acids Res 2014; 42:D206–D214 [View Article]
     [Google Scholar]
  32. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article] [PubMed]
     [Google Scholar]
  33. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007; 57:81–91 [View Article] [PubMed]
     [Google Scholar]
  34. Na S-. I, Kim YO, Yoon S-. H, Ha S-. M, Baek I et al. UBCG: Up-to-date bacterial core gene set and pipeline for phylogenomic tree reconstruction. J Microbiol 2018; 56:281–285
     [Google Scholar]
  35. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 2009; 106:19126–19131 [View Article] [PubMed]
     [Google Scholar]
  36. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. Report of the ad hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Int J Syst Bacteriol 1987; 37:463–464
     [Google Scholar]
  37. Burg MB, Ferraris JD. Intracellular organic osmolytes: function and regulation. J Biol Chem 2008; 283:7309–7313 [View Article] [PubMed]
     [Google Scholar]
  38. Mongodin EF, Nelson KE, Daugherty S, DeBoy RT, Wister J et al. The genome of Salinibacter ruber: convergence and gene exchange among hyperhalophilic bacteria and archaea. Proc Natl Acad Sci U S A 2005; 102:18147–18152 [View Article] [PubMed]
     [Google Scholar]
  39. Wommack KE, Colwell RR. Virioplankton: viruses in aquatic ecosystems. Microbiol Mol Biol Rev 2000; 64:69–114 [View Article] [PubMed]
     [Google Scholar]
  40. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
     [Google Scholar]
  41. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241
     [Google Scholar]
  42. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–207
     [Google Scholar]
  43. Li G, Lai Q, Dong C, Ma R, Du Y et al. Roseovarius atlanticus sp. nov., isolated from surface seawater. Int J Syst Evol Microbiol 2016; 66:639–644 [View Article] [PubMed]
     [Google Scholar]
  44. Liang Q-Y, Xu Z-X, Zhang J, Chen G-J, Du Z-J. Salegentibacter sediminis sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from coastal sediment. Int J Syst Evol Microbiol 2018; 68:2375–2380 [View Article] [PubMed]
     [Google Scholar]
  45. Yoon J-H, Jung S-Y, Kang S-J, Jung Y-T, Oh T-K. Salegentibacter salarius sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 2007; 57:2738–2742 [View Article] [PubMed]
     [Google Scholar]
  46. Nedashkovskaya OI, Kim SB, Lysenko AM. Salegentibacter mishustinae sp. nov., isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 2005; 55:235–238 [View Article] [PubMed]
     [Google Scholar]
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Mesonia hitae sp. nov., isolated from the seawater of the South Atlantic Ocean
Int J Syst Evol Microbiol 71, 004911 (2021); https://doi.org/10.1099/ijsem.0.004911
/content/journal/ijsem/10.1099/ijsem.0.004911
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