1887

Abstract

A novel Gram-stain-negative, aerobic and rod-shaped bacterial strain designated as 6D45A was isolated from mangrove soil and characterized using a polyphasic taxonomic approach. Strain 6D45A was found to grow at 10–37 °C (optimum, 28 °C), at pH 6.0–9.0 (optimum, 7.0) and in 0–5 % (w/v) NaCl (optimum, 2%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 6D45A fell into the genus and shared 99.1 % identity with the closest type strain CGMCC 1.3455 and less than 97.2 % identity with other type strains of this genus. The 34.8 % digital DNA–DNA hybridization (dDDH) and 88.3 % average nucleotide identity (ANI) values between strain 6D45A and the closest relative above were well below recognized thresholds of 70 % DDH and 95–96 % ANI for species definition, implying that strain 6D45A should represent a novel genospecies. The phylogenomic analysis indicated that strain 6D45A formed an independent branch distinct from reference strains. The predominant cellular fatty acid of strain 6D45A was summed feature 8 (C c and/or C c, 66.9 %); the polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, two unidentified glycolipids and an unknown lipid; the respiratory quinone was Q-10. The genomic DNA G+C content was 66.5 mol %. Based on the phenotypic and genotypic characteristics, strain 6D45A is concluded to represent a novel species of the genus , for which the name sp. nov., is proposed. The type strain of the species is 6D45A (=GDMCC 1.1960=KCTC 82334). We also propose the reclassification of as comb. nov. and ‘’ as a species of the genus .

Funding
This study was supported by the:
  • The Science and Technology Plan Project of Guangdong Province (Award 2019B030316017)
    • Principle Award Recipient: Ming-RongDeng
  • The GDAS' Project of Science and Technology Development (Award 2020GDASYL-20200103028)
    • Principle Award Recipient: JuanDu
  • The Key-Area Research and Development Program of Guangdong Province (Award 2018B020205003)
    • Principle Award Recipient: HonghuiZhu
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2021-07-16
2021-08-04
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