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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 7

gen. nov., sp. nov., represented by a marine strain of the family Free

Abstract

A Gram-stain-negative, non-motile, facultatively anaerobic, short rod-shaped bacterium, designated HB171799, was isolated from seacoast sandy soil collected at Qishui Bay, Hainan, PR China. The chemotaxonomic analysis revealed that the respiratory quinones were Q-8 and Q-7, and the major cellular fatty acids were summed feature 8 (comprising C c and/or C c), C and C. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified lipid. The size of the draft genome was 3.68 Mb with a DNA G+C content of 48.0 mol%. Results of phylogenetic analyses based on 16S rRNA gene and genome sequences showed that the novel isolate belonged to the family and formed a distinct subcluster at the base of the radiation of the genus . The highest sequence similarity (96.0 %) of the novel isolate was found to the type strains of JCM 18476 and DSM23425. The whole genome-based phylogeny and differences in cellular fatty acids and polar lipids readily distinguished strain HB171799 from all the closely related validly published type strains. Strain HB171799 is therefore suggested to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is HB171799 (=CGMCC 1.16727=JCM 33332).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , Maribrevibacterium harenarium , Oceanospirillaceae and polyphasic taxonomy
Funding
This study was supported by the:
  • Central Public-interest Scientific Institution Basal Research Fund of CATAS from Chinese Government (Award 1630052019014)
    • Principle Award Recipient: HuiqinHuang
  • Central Public-interest Scientific Institution Basal Research Fund of CATAS from Chinese Government (Award 1630052016011)
    • Principle Award Recipient: HuiqinHuang
  • Financial Fund of Ministry of Agriculture and Rural Affairs of China (Award NFZX2021)
    • Principle Award Recipient: YonghuaHu
  • Financial Fund of Ministry of Agriculture and Rural Affairs of China (Award NHYYSWZZZYKZX2020)
    • Principle Award Recipient: YonghuaHu
© 2021 The Authors
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2021-07-29
2024-12-02
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Maribrevibacterium harenarium gen. nov., sp. nov., represented by a marine strain of the family Oceanospirillaceae
Int J Syst Evol Microbiol 71, 004872 (2021); https://doi.org/10.1099/ijsem.0.004872
/content/journal/ijsem/10.1099/ijsem.0.004872
/content/journal/ijsem/10.1099/ijsem.0.004872
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