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Abstract

One thermophilic bacterium, designated strain SYSU G02662, was isolated from hot spring sediment sampled in Tibet, PR China. Polyphasic taxonomic analyses and whole-genome sequencing were used to determine the taxonomy position of the strain. Phylogenetic analysis using 16S rRNA gene sequences indicated that strain SYSU G02662 showed the highest sequence similarity to SCSIO 58843 (95.1 %). The strain could be differentiated from other species of the family by its distinct phenotypic and genotypic characteristics. Cells of strain SYSU G02662 were aerobic, Gram-staining-positive and short rodshaped. Growth occurred optimally at 45 °C and pH 7.0. In addition, -diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The respiratory quinone was MK-9 (H), while the major fatty acids (>10 %) were C, C, C and iso-C. The detected polar lipids included diphosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylinositol. The G+C content of the genomic DNA was 70.5 % based on the draft genomic sequence. On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU G02662 represents a novel species of a novel genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the proposed novel species is SYSU G02662 (=CGMCC 4.7688=KCTC 49500).

Funding
This study was supported by the:
  • Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) (Award 3110200005)
    • Principle Award Recipient: Wen-JunLi
  • National Natural Science Foundation of China (Award 31670009)
    • Principle Award Recipient: Wen-JunLi
  • National Natural Science Foundation of China (Award 91951205)
    • Principle Award Recipient: Wen-JunLi
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2021-03-19
2021-10-17
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