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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 3

sp. nov., a novel hydrogen- and sulfur-oxidizing chemolithoautotroph isolated from a deep-sea hydrothermal plume in the Northwestern Indian Ocean Free

Abstract

A novel mesophilic, hydrogen-, and sulfur-oxidizing bacterium, designated strain ST-419, was isolated from a deep-sea hydrothermal vent plume on the Carlsberg Ridge of the Northwestern Indian Ocean. The isolate was a Gram-staining-negative, non-motile and coccoid to oval-shaped bacterium. Growth was observed at 4–50 °C (optimum 37 °C), pH 5.0–8.6 (optimum pH 6.0) and 1.0–5.0 % (w/v) NaCl (optimum 3.0 %). ST-419 could grow chemlithoautotrophically with molecular hydrogen, sulfide, elemental sulfur and thiosulfate as energy sources. Molecular oxygen, nitrate and elemental sulfur could be used as electron acceptors. The predominant fatty acids were Cω7, Cω7 and C. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The respiratory quinone was menaquinone MK-6 and the G+C content of the genomic DNA was 42.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that ST-419 represented a member of genus and was most closely related to 1812E, with 97.6 % sequence similarity. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between ST-419 and 1812E were 74.6 and 19.6 %, respectively. The combined genotypic and phenotypic data indicate that ST-419 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is ST-419 (=MCCC 1A17954=KCTC 25164).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): hydrothermal vent , sulfur-oxidizing and Sulfurovum indicum
Funding
This study was supported by the:
  • China Ocean Mineral Resources R & D Association (Award No. DY135-S2-01)
    • Principle Award Recipient: NotApplicable
  • China Ocean Mineral Resources Research and Development Association (Award No. DY135-B2-01)
    • Principle Award Recipient: NotApplicable
  • National Natural Science Foundation of China (Award No.91951201)
    • Principle Award Recipient: NotApplicable
  • National Natural Science Foundation of China (Award No. 41672333)
    • Principle Award Recipient: NotApplicable
  • National Key R&D Program of China (Award 2018YFC0310701)
    • Principle Award Recipient: NotApplicable
© 2021 The Authors
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2021-03-18
2024-04-26
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Sulfurovum indicum sp. nov., a novel hydrogen- and sulfur-oxidizing chemolithoautotroph isolated from a deep-sea hydrothermal plume in the Northwestern Indian Ocean
Int J Syst Evol Microbiol 71, 004748 (2021); https://doi.org/10.1099/ijsem.0.004748
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