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Volume 71, Issue 3

sp. nov., isolated from humic soil on bird’s nest fern ( L.) Free

Abstract

A novel member of the actinobacteria, designated strain A-T 0013, was isolated from humic soil on a bird’s nest fern ( L.) collected from Khao Yai National Park in Thailand. According the results of a polyphasic taxonomic study, A-T 0013 had characteristics typical of members of the genus . The 16S rRNA gene sequence indicated that A-T 0013 shared ≤98 % sequence similarity with all members of the genus . The most closely related species was IFM 10200 (97.92 % sequence similarity). The average nucleotide identity based on (ANIb) value with IFM 10200 was 76.81 %. The cell-wall peptidoglycan contained -diaminopimelic acid. The whole-cell sugars contained ribose, arabinose and galactose. The predominant menaquinone was MK-9(H). The predominant fatty acids were C, Cω9, summed feature 3 (Cω7/Cω6), and C 10-methyl. Mycolic acid was present. The polar lipid profile for this strain ncluded diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The G+C content of the genomic DNA was 66.1 mol%. Differentiation of A-T 0013 from the most closely related species, IFM 10200, was evident from digital DNA–DNA hybridization values of 21.8 %. On the basis of the results of comparative analysis of phenotypic, chemotaxonomic and genotypic data, strain A-T 0013 is concluded to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A-T 0013 (=TBRC 11910=NBRC 114549).

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Keyword(s): bird’s nest fern , Gordonia asplenii and humic soil
© 2021 The Authors
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2021-03-12
2024-05-05
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Gordonia asplenii sp. nov., isolated from humic soil on bird’s nest fern (Asplenium nidus L.)
Int J Syst Evol Microbiol 71, 004746 (2021); https://doi.org/10.1099/ijsem.0.004746
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