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Volume 71, Issue 3

sp. nov., isolated from Antarctic tundra soil, and emended descriptions of , , and Free

Abstract

A rod-shaped, yellow-pigmented, Gram-stain-negative, non-motile and aerobic bacterium, designated 7-3A, was isolated from soil from King George Island, maritime Antarctica, and subjected to a polyphasic taxonomic study. Growth occurred at 4–37 °C (optimum, 20°C) and at pH 5.0–9.0 (optimum, pH 7.0–8.0). Tolerance to NaCl was up to 4 % (w/v) with optimum growth in the absence of NaCl. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 7-3A represented a member of the family . Strain 7-3A showed the highest sequence similarities with HMD 1043 (96.65 %), NCTC 13525 (96.53 %), DSM 23145 (96.27 %), LMG 24720 (96.13 %) and DSM 17048 (96.06 %). A whole genome-level comparison of 7-3A with DSM 17048, LMG 24720, DSM 23145, and DSM 21579 revealed average nucleotide identity (ANI) values of 79.03, 82.25, 78.12, and 74.42 %, respectively. The major respiratory isoprenoid quinone was identified as MK-6 and a few ubiquinones Q-10 were identified. In addition, flexirubin-type pigments were absent. The polar lipid profile of 7-3A was found to contain one phosphatidylethanolamine, six unidentified aminolipids (AL) and two unidentified lipids (L). The G+C content of the genomic DNA was determined to be 34.54 mol%. The main fatty acids were iso-C, summed feature 9 (comprising iso-Cω9 and/or C 10-methyl), anteiso-C, iso-C and summed feature 3 (comprising Cω7 and/or Cω6). On the basis of the evidence presented in this study, a novel species of the genus , sp. nov., is proposed, with the type strain 7-3A (=CCTCC AB 2016141= KCTC 52492). Emended descriptions of , , and are also given.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Kaistella flava , novel species , phylogenetic analysis and taxonomy
Funding
This study was supported by the:
  • the Chinese Polar Scientific Strategy Research Fund (Award IC201706)
    • Principle Award Recipient: FangPeng
  • the National Natural Science Foundation of China (Award Grant No. 42076230)
    • Principle Award Recipient: FangPeng
  • the R&D Infrastructure and Facility Development Program of the Ministry of Science and Technology of the People’s Republic of China (Award Grant No. NIMR-2020-8)
    • Principle Award Recipient: FangPeng
  • the National Key R&D Program of China (Award 2018YFC1406701)
    • Principle Award Recipient: FangPeng
© 2021 The Authors
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2021-03-16
2024-04-26
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Kaistella flava sp. nov., isolated from Antarctic tundra soil, and emended descriptions of Kaistella yonginensis, Kaistella jeonii, Kaistella antarctica and Kaistella chaponensis
Int J Syst Evol Microbiol 71, 004740 (2021); https://doi.org/10.1099/ijsem.0.004740
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