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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 71, Issue 3

sp. nov. from of Hainan island Free

Abstract

Two strains (NLN63 and NLN82) of Gram-stain-negative, oxidase- and catalase-positive, bacilli-shaped organisms were isolated from the faecal samples of two separate in Baisha county of Hainan Province, Southern PR China. Phylogenetic analysis based on the near full-length 16S rRNA sequences revealed that strain NLN63 belongs to the genus , having maximum similarity to CCUG 64465 (97.1 %), CCUG 39967 (96.2 %) and DSM 27484 (96.2 %), respectively. The phylogenomic tree built on 553 core genes from genomes of 20 species in the genus and other adjacent genera further confirmed that strains NLN63 and NLN82 form a distinct subline and exhibit specific phylogenetic affinity with CCUG 39967. In digital DNA–DNA hybridization analyses, strain NLN63 showed low estimated DNA reassociation values (21.4–22.6 %) with the type strains of the species in the genus . The DNA G+C contents of strains NLN63 and NLN82 were 37.3 and 37.1 mol%, respectively. Strain NLN63 had a unique MALDI-TOF MS profile, contained Q-8 as the major quinone and C, summed feature 8 (C 7/C 6 or both) and summed feature 3 (C 7/C 6 or both) as the dominant fatty acids. Based upon these polyphasic characterization data obtained from the present study, a novel species of the genus , sp. nov., is proposed with NLN63 (=GDMCC 1.1697=JCM 33788) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA , Hainan island , Pelistega ratti and Rattus norvegicus
Funding
This study was supported by the:
  • the Innovative research project for graduate students of Hainan Medical University (Award HYYS2020-18)
    • Principle Award Recipient: DuanduanXuan
  • Key R&D Programs of Hainan Province (Award ZDYF2018113)
    • Principle Award Recipient: XiaojunZhou
  • the Key Science and Technology Projects of Hainan Province (Award ZDYF2018140)
    • Principle Award Recipient: XiaojunZhou
  • the Finance science and technology project of Hainan Privince (Award ZDYF2018155)
    • Principle Award Recipient: XiaojunZhou
  • the National Natural Science Foundation of China (Award 82060377, 81760376)
    • Principle Award Recipient: LinaNiu
  • Education Department of Hainan Province (CN) (Award Hnky2019ZD-27)
    • Principle Award Recipient: LinaNiu
  • Hainan Provincial Natural Science Foundation of China (Award 820RC650)
    • Principle Award Recipient: LinaNiu
© 2021 The Authors
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2021-03-10
2025-01-21
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Pelistega ratti sp. nov. from Rattus norvegicus of Hainan island
Int J Syst Evol Microbiol 71, 004733 (2021); https://doi.org/10.1099/ijsem.0.004733
/content/journal/ijsem/10.1099/ijsem.0.004733
/content/journal/ijsem/10.1099/ijsem.0.004733
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