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Volume 71, Issue 3

sp. nov., isolated from roots Free

Abstract

A Gram-stain-variable, aerobic, rod-shaped, endospore-forming strain R196) was isolated from internal tissues of roots of . Cells were motile with peritrichous flagella. The colonies were light pink on tryptone soya agar medium. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain R196 fell into a phylogenetic cluster belonging to the genus . Strain R196 was closely related to C4-5 and CKOBP-6 with 93.6 and 93.3% sequence similarities, respectively. The major cellular polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids, two unidentified aminophospholipids and an unidentified aminolipid. The dominant respiratory quinone was MK-7. The main cellular fatty acids were anteiso-C (53.01%), C (13.04%) and iso-C (10.80%). The genome size of R196 was 9.45 Mb, containing 7617 predicted protein-coding genes, with a DNA G+C content of 57.7 mol%. Based on the results of phenotypic, chemotaxonomic and whole-genome analyses, strain R196 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R196 (=ACCC 61713=KCTC 33718).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Cymbidium goeringii , Paenibacillus cymbidii sp. nov and root endophyte
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award No. 31100002)
    • Principle Award Recipient: LeiSun
© 2021 The Authors
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2021-03-05
2024-04-26
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Paenibacillus cymbidii sp. nov., isolated from Cymbidium goeringii roots
Int J Syst Evol Microbiol 71, 004731 (2021); https://doi.org/10.1099/ijsem.0.004731
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