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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 3

Elevation of subsp. to the species level as sp. nov. and transfer of subsp. to as subsp. comb. nov. Free

Abstract

Currently, contains four subspecies: subsp. , subsp. , subsp. and subsp. . In the study of Pérez , these four subspecies could be clearly divided into two groups based on sequence analysis: subsp. and subsp. ; subsp. and subsp. . The two groups had a relatively low DNA–DNA hybridization value (about 60 %). In the present study, the taxonomic position of subsp. and subsp. was re-examined based on sequence analyses of 16S rRNA, , and genes, average nucleotide identity (ANI) values and digital DNA–DNA hybridization (dDDH) values. The result of 16S rRNA gene sequence analysis indicated that subsp. NCDO 607 and subsp. L105 were phylogenetically related to the type strains of subsp. , subsp. , , , , , , and . The 16S rRNA gene, , , and concatenated , and sequence similarities, ANI values, and dDDH values between the type strains of subsp. , subsp. and phylogenetically related species were 93.5–99.4 %, 83.3–97.6 %, 80.6–92.4 %, 79.7–92.7 %, 83.5–94.3 %, 72.4–86.9 % and 21.4–32.5 %, respectively. Lower than 95–96 % ANI values and lower than 70 % dDDH values confirmed that the type strains of subsp. and subsp. represent a novel species in the genus . Because subsp. was proposed and validated before subsp. , subsp. is elevated to the species level as sp. nov. and subsp. is transferred to as subsp. comb. nov. The type strain of sp. nov. is NCDO 607 (=ATCC 19257=DSM 20069=JCM 16167=LMG 6897=NBRC 100676). The type strain of subsp. comb. nov. is L105 (=NBRC 110453=DSM 21502=JCM 31125=LMG 24662).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genome , Lactococcus and reclassification
Funding
This study was supported by the:
  • Agricultural Science and Technology Innovation Program, China (ASTIP) and the Building of Modern Agricultural Industry (Bees) R&D Systems in China (Award NYCYTI-43-KXJ17)
    • Principle Award Recipient: WenLi Tian
  • National Natural Science Foundation of China (Award no. 31972087)
    • Principle Award Recipient: WenLi Tian
  • National Natural Science Foundation of China (Award no. 31471594)
    • Principle Award Recipient: ChunTao Gu
© 2021 The Authors
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2021-03-02
2024-04-26
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Elevation of Lactococcus lactis subsp. cremoris to the species level as Lactococcus cremoris sp. nov. and transfer of Lactococcus lactis subsp. tructae to Lactococcus cremoris as Lactococcus cremoris subsp. tructae comb. nov.
Int J Syst Evol Microbiol 71, 004727 (2021); https://doi.org/10.1099/ijsem.0.004727
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