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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 3

sp. nov., an actinobacterium isolated from cow dung Free

Abstract

A novel actinobacterium, designated strain NEAU-351, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, northeast PR China and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-351 belonged to the genus , with the highest similarity (98.96 %) to DSM 44801 and less than 98.0 % identity with other type strains of the genus . The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major menaquinone was observed to contain MK-8(H, ω-cycl) (78.2 %). The fatty acid profile mainly consisted of C, C 9 and 10-methyl C. Mycolic acids were present. The genomic DNA G+C content of strain NEAU-351 was 68.1 mol%. In addition, the average nucleotide identity values between strain NEAU-351 and its reference strains, DSM 44801 and NBRC 108935, were found to be 81.4 and 82.9 %, respectively, and the level of digital DNA–DNA hybridization between them were 24.8 % (22.5–27.3 %) and 26.3 % (24–28.8 %), respectively. Here we report on the taxonomic characterization and classification of the isolate and propose that strain NEAU-351 represents a new species of the genus , for which the name is proposed. The type strain is NEAU-351 (=CCTCC AA 2019090=DSM 110681).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene sequence , cow dung , Nocardia bovistercoris sp. nov. and polyphasic analysis
Funding
This study was supported by the:
  • the National Natural Science Foundation of China (Award 31872037)
    • Principle Award Recipient: WenshengXiang
© 2021 The Authors
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2021-02-09
2024-05-05
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Nocardia bovistercoris sp. nov., an actinobacterium isolated from cow dung
Int J Syst Evol Microbiol 71, 004699 (2021); https://doi.org/10.1099/ijsem.0.004699
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