1887

Abstract

This study presents taxonomic descriptions of strains CYK-4 and TWA-26 isolated from freshwater habitats in Taiwan. Both strains were Gram-stain-negative, strictly aerobic, motile by gliding and rod-shaped. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that both strains belonged to the genus . Analysis of 16S rRNA gene sequences showed that strains CYK-4 and TWA-26 shared 92.7 % sequence similarity and were most closely related to W201E (95.6 %) and JC164 (96.7 %), respectively. Both strains shared common chemotaxonomic characteristics comprising MK-6 as the main isoprenoid quinone, iso-C and iso-C G as the predominant fatty acids, phosphatidylethanolamine as the principal polar lipid, and homospermidine as the major polyamine. The DNA G+C contents of strains CYK-4 and TWA-26 were 41.5 and 31.8 mol%, respectively. The average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between these two novel isolates and their closest relatives were below the cut-off values of 95–96, 90 and 70 %, respectively, used for species demarcation. On the basis of phenotypic and genotypic properties and phylogenetic inference, both strains should be classified as novel species within the genus , for which the names sp. nov. (type strain CYK-4=BCRC 81192=LMG 31330) and sp. nov. (type strain TWA-26=BCRC 81200=LMG 31333) are proposed.

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2021-02-08
2021-10-19
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