1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 3

sp. nov., a lactic acid bacterium isolated from animal feed Free

Abstract

A Gram-positive, facultative anaerobic, catalase-negative, non-motile, non-spore-forming and rod-shaped lactic acid bacterium strain, denoted as NFFJ11 and isolated from total mixed fermentation feed in the Republic of Korea, was characterized through polyphasic approaches, including sequence analyses of the 16S rRNA gene and housekeeping genes ( and ), determination of average nucleotide identity and DNA–DNA hybridization, fatty acid methyl ester analysis, and phenotypic characterization. Phylogenetic analyses based on 16S rRNA, and gene sequences revealed that strain NFFJ11 belonged to the genus . The 16S rRNA gene sequence of strain NFFJ11 exhibited high similarity to S215 (99.66 %), Rv4 na (99.53 %), LMG 23699 (99.19 %), YM 0097 (99.06 %), HBUAS52074 (98.86 %) and S4-3 (98.66 %). However, average nucleotide identity and DNA–DNA hybridization values for these type strains were in the range of 79.90–92.93 % and 23.80–49.30 %, respectively, which offer evidence that strain NFFJ11 belongs to a novel species of the genus . The cell-wall peptidoglycan type was A4α (-Lys-Asp) and the G+C content of the genomic DNA was 35.7 mol%. The main fatty acids of strain NFFJ11 were C 9 (43.3 %), C (20.1 %) and summed feature 7 (18.3 %; comprising any combination of C 7, C 6 and C cyclo 10). Through polyphasic taxonomic analysis, it was observed that strain NFFJ11 represents a novel species belonging to the genus , for which the name sp. nov. is proposed. The type strain is NFFJ11 (= KACC 21771= JCM 34088).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): animal feed , Companilactobacillus pabuli sp. nov. and new species
© 2021 The Authors
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004670
2021-01-27
2024-05-11
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/71/3/ijsem004670.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.004670&mimeType=html&fmt=ahah

References

  1. Chen YS, Wang LT, Liao YJ, Lan YS, Chang CH et al. Lactobacillus musae sp. nov., a novel lactic acid bacterium isolated from banana fruits. Int J Syst Evol Microbiol 2017; 67:5144–5149 [View Article][PubMed]
     [Google Scholar]
  2. McFrederick QS, Vuong HQ, Rothman JA. Lactobacillus micheneri sp. nov., Lactobacillus timberlakei sp. nov. and Lactobacillus quenuiae sp. nov., lactic acid bacteria isolated from wild bees and flowers. Int J Syst Evol Microbiol 2018; 68:1879–1884 [View Article][PubMed]
     [Google Scholar]
  3. Lee SH, Park MS, Jung JY, Jeon CO. Leuconostoc miyukkimchii sp. nov., isolated from brown algae (Undaria pinnatifida) kimchi. Int J Syst Evol Microbiol 2012; 62:1098–1103 [View Article][PubMed]
     [Google Scholar]
  4. Chiou TY, Suda W, Oshima K, Hattori M, Matsuzaki C et al. Lactobacillus kosoi sp. nov., a fructophilic species isolated from kôso, a Japanese sugar-vegetable fermented beverage. Antonie van Leeuwenhoek 2018; 111:1149–1156 [View Article][PubMed]
     [Google Scholar]
  5. Tohno M, Tanizawa Y, Irisawa T, Masuda T, Sakamoto M et al. Lactobacillus silagincola sp. nov. and Lactobacillus pentosiphilus sp. nov., isolated from silage. Int J Syst Evol Microbiol 2017; 67:3639–3644 [View Article][PubMed]
     [Google Scholar]
  6. Goyal R, Dhingra H. Isolation and identification of genus Lactobacillus from different curd samples. Biosci Biotechnol Res Asia 2010; 7:907–912
     [Google Scholar]
  7. Beijerinck MW. Sur les ferments lactiques de l’industrie. Archiv Néerlandaises des Sciences Exactes et Naturelles 1901; 6:212–243
     [Google Scholar]
  8. Parte AC. LPSN -list of prokaryotic names with standing in nomenclature (bacterio.net), 20 years on. Int J Syst Evol Microbiol 2018; 68:1825–1829 [View Article][PubMed]
     [Google Scholar]
  9. Zheng J, Wittouck S, Salvetti E, Franz CMAP, Harris HMB et al. A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae . Int J Syst Evol Microbiol 2020; 70:2782–2858 [View Article][PubMed]
     [Google Scholar]
  10. Kim JM, Le NT, Chung BS, Park JH, Bae J-W et al. Influence of soil components on the biodegradation of benzene, toluene, ethylbenzene, and o-, m-, and p-xylenes by the newly isolated bacterium Pseudoxanthomonas spadix BD-a59. Appl Environ Microbiol 2008; 74:7313–7320 [View Article][PubMed]
     [Google Scholar]
  11. Yoon SH, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  12. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22:4673–4680 [View Article][PubMed]
     [Google Scholar]
  13. Kumar S, Stecher G, Tamura K. mega7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
     [Google Scholar]
  14. Chang CH, Chen YS, Lee TT, Chang YC, Yu B. Lactobacillus formosensis sp. nov., a lactic acid bacterium isolated from fermented soybean meal. Int J Syst Evol Microbiol 2015; 65:101–106 [View Article][PubMed]
     [Google Scholar]
  15. Chao SH, Kudo Y, Tsai YC, Watanabe K. Lactobacillus futsaii sp. nov., isolated from fu-tsai and suan-tsai, traditional Taiwanese fermented mustard products. Int J Syst Evol Microbiol 2012; 62:489–494 [View Article][PubMed]
     [Google Scholar]
  16. Reuter G. Lactobacillus alimentarius sp. nov., nom rev. and Lactobacillus farciminis sp. nov., nom. rev. Syst Appl Microbiol 1983; 4:277–279 [View Article][PubMed]
     [Google Scholar]
  17. Scheirlinck I, Van der Meulen R, Van Schoor A, Huys G, Vandamme P et al. Lactobacillus crustorum sp. nov., isolated from two traditional Belgian wheat sourdoughs. Int J Syst Evol Microbiol 2007; 57:1461–1467 [View Article][PubMed]
     [Google Scholar]
  18. Zhang Z, Hou Q, Wang Y, Li W, Zhao H et al. Lactobacillus zhachilii sp. nov., a lactic acid bacterium isolated from Zha-Chili. Int J Syst Evol Microbiol 2019; 69:2196–2201 [View Article][PubMed]
     [Google Scholar]
  19. Gu CT, Li CY, Yang LJ, Huo GC. Lactobacillus heilongjiangensis sp. nov., isolated from Chinese pickle. Int J Syst Evol Microbiol 2013; 63:4094–4099 [View Article][PubMed]
     [Google Scholar]
  20. Kim M, Oh H-S, Park S-C, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [View Article][PubMed]
     [Google Scholar]
  21. Jung JY, Han S-S, Kim Z-H, Ryu BG, Jin HM et al. Complete genome sequence of Lactobacillus harbinensis strain NSMJ42, isolated from makgeolli, a traditional Korean alcoholic beverage. Microbiol Resour Announc 2019; 8:e01177–19 [View Article][PubMed]
     [Google Scholar]
  22. Chin CS, Alexander DH, Marks P, Klammer AA, Drake J et al. Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nat Methods 2013; 10:563–569 [View Article][PubMed]
     [Google Scholar]
  23. Kurtz S, Phillippy A, Delcher AL, Smoot M, Shumway M et al. Versatile and open software for comparing large genomes. Genome Biol 2004; 5:R12 [View Article][PubMed]
     [Google Scholar]
  24. Yoon SH, Ha S-M, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017; 110:1281–1286 [View Article][PubMed]
     [Google Scholar]
  25. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461–466 [View Article][PubMed]
     [Google Scholar]
  26. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article][PubMed]
     [Google Scholar]
  27. Thompson CC, Chimetto L, Edwards RA, Swings J, Stackebrandt E et al. Microbial genomic taxonomy. BMC Genomics 2013; 14:913 [View Article][PubMed]
     [Google Scholar]
  28. Besemer J, Lomsadze A, Borodovsky M. GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions. Nucleic Acids Res 2001; 29:2607–2618 [View Article][PubMed]
     [Google Scholar]
  29. Konstantinidis KT, Tiedje JM. Towards a genome-based taxonomy for prokaryotes. J Bacteriol 2005; 187:6258–6264 [View Article]
     [Google Scholar]
  30. da X, Jiang F, Chang X, Ren L, Qiu X. Pedobacter ardleyensis sp. nov., isolated from soil of the Ardley Island, South Shetland Islands in Antarctica. Int J Syst Evol Microbiol 2015; 65:3841–3846
     [Google Scholar]
  31. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp 607–654
     [Google Scholar]
  32. Schleifer KH, Kandler O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 1972; 36:407–477 [View Article][PubMed]
     [Google Scholar]
  33. Miller LT. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters. including hydroxy acid. J Clin Microbiol 1982; 18:861–867
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004670
Loading
Companilactobacillus pabuli sp. nov., a lactic acid bacterium isolated from animal feed
Int J Syst Evol Microbiol 71, 004670 (2021); https://doi.org/10.1099/ijsem.0.004670
/content/journal/ijsem/10.1099/ijsem.0.004670
/content/journal/ijsem/10.1099/ijsem.0.004670
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error