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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 2

sp. nov. and sp. nov., isolated from plateau pika () on the Qinghai–Tibet Plateau of PR China Free

Abstract

Four novel bacterial strains (ST-M6, L-033, L-031 and Z-333) were isolated from the intestinal contents of plateau pikas () collected on the QinghaiTibet Plateau, PR China. Cells were aerobic, non-motile, Gram-stain-positive, catalase-positive, oxidase-negative, capsuled and short-rod-shaped. Phylogenetic analyses based on the 16S rRNA gene sequences and 387 core genes indicated that the four isolates belong in the genus and clearly separate from recognized species. The two type strains (ST-M6 and L-031) shared low 16S rRNA similarity, average nucleotide identity values and digital DNA–DNA hybridization relatedness with their phylogenetic neighbours ( DSM 18659, DSM 19179, JCM 30598, CCTCC M208212, DSM 16091 and DSM 20754). The genomic DNA G+C contents of strains ST-M6 and L-031 were 70.4 and 70.7 mol%, respectively. The major cellular fatty acids of strain ST-M6 were anteiso-C, anteiso-C and iso-C, in contrast to anteiso-C, anteiso-C and anteiso-C 9 of strain L-031. Both type strains (ST-M6 and L-031) were glycolate test positive and shared the following common features: MK-11 and MK-12 as major menaquinones; rhamnose, ribose, mannose and galactose as major cell-wall sugars; diphosphatidylglycerol, phosphatidylglycerol and two glycolipids as polar lipids; and ornithine, alanine, glycine and glutamic acid as cell-wall amino acids. Comparing the phenotypic, phylogenetic and chemotaxonomic features of the four strains and their related taxa, strains ST-M6 and L-031 represent two novel species of the genus , for which the names sp. nov. (type strain ST-M6=CGMCC 1.16364=DSM 104058) and sp. nov. (type strain L-031 =CGMCC 1.16363=DSM 106170) are proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Microbacterium caowuchunii , Microbacterium lushaniae , Ochotona curzoniae , plateau pika and Qinghai–Tibet Plateau
Funding
This study was supported by the:
  • Research Units of Discovery of Unknown Bacteria and Function (Award 2018RU010)
    • Principle Award Recipient: JianguoXu
  • Sanming Project of Medicine in Shenzhen (Award SZSM201811071)
    • Principle Award Recipient: JianguoXu
  • National Key R&D Program of China (Award 2018YFC1200102)
    • Principle Award Recipient: DongJin
  • National Science and Technology Major Project of China (Award 2018ZX10712001-018)
    • Principle Award Recipient: NotApplicable
  • National Science and Technology Major Project of China (Award 2018ZX10712001-007)
    • Principle Award Recipient: JingYang
© 2021 The Authors
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2021-01-27
2024-05-06
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Microbacterium caowuchunii sp. nov. and Microbacterium lushaniae sp. nov., isolated from plateau pika (Ochotona curzoniae) on the Qinghai–Tibet Plateau of PR China
Int J Syst Evol Microbiol 71, 004662 (2021); https://doi.org/10.1099/ijsem.0.004662
/content/journal/ijsem/10.1099/ijsem.0.004662
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