1887

Abstract

A bisphenol A-degrading bacterium, designated as strain H4, was isolated from surface seawater, which was sampled from the Jiulong River estuary in southeast PR China. Strain H4 is Gram-stain-negative, aerobic, short rod-shaped, lacking bacteriochlorophyll a, motile with multifibrillar stalklike fascicle structures and capable of degrading bisphenol A. Growth of strain H4 was observed at 24–45 °C (optimum, 32 °C), at pH 5.5–9 (optimum, pH 7.0) and in 0–7 % NaCl (optimum, 2 %; w/v) . The 16S rRNA gene sequence of strain H4 showed highest similarity to Ery9 (98.7 %), (98.3 %), PQ-2 (98.1 %) and GM-16 (97.6 %), followed by E4A9 (96.7 %) and Ery22 (96.0 %). Phylogenetic analysis revealed that strain H4 fell within a clade comprising the type strains of species and formed a phyletic line with them that was distinct from other members of the family . The sole respiratory quinone was quinone 10 (Q-10). The predominant fatty acids (>5 % of the total fatty acids) of strain H4 were summed feature 8 (C 6 and/or C 7), summed feature 3 (C 6 and/or C 7), C 6 and C2-OH. The genomic DNA G+C content was 62.8 mol%. In the polar lipid profile, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids, two sphingoglycolipids and three unknown lipids were the major compounds. Based on the genotypic and phenotypic data, strain H4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is H4 (=DSM 102182=MCCC1 K02301).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41673099)
    • Principle Award Recipient: Chang-PingYu
  • National Natural Science Foundation of China (Award 31500420)
    • Principle Award Recipient: JiangweiLi
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2021-01-25
2021-10-16
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