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Volume 71, Issue 2

gen. nov., sp. nov., a microaerophilic sulfur-oxidizing bacterium isolated from freshwater sediment Free

Abstract

A novel sulfur-oxidizing bacterium, designated strain LSR1, was enriched and isolated from a freshwater sediment sample collected from the Pearl River in Guangzhou, PR China. The strain was an obligate chemolithoautotroph, using thiosulfate or sulfide as an electron donor and energy source. Growth of strain LSR1 was observed at 15–40 °C, pH 6.0–7.5 and NaCl concentrations of 0–1.5 %. Strain LSR1 was microaerophilic, with growth only at oxygen content less than 10 %. Anaerobic growth was also observed when using nitrate as the sole electron acceptor. The major cellular fatty acids were C and summed feature 3 (comprising C ω7 and/or C ω6). The DNA G+C content of the draft genome sequence was 67.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LSR1 formed a lineage within the family , showing sequence identities of 92.87, 92.33 and 90.80 % with its closest relative genera , and , respectively. The genome of strain LSR1 contained multiple genes encoding sulfur-oxidizing enzymes that catalyse thiosulfate and sulfide oxidation, and the gene encoding -type cytochrome c oxidase and -type quinol oxidase, which enables strain LSR1 to perform sulphur oxidation under microaerophilic conditions. On the basis of phenotypic, genotypic and phylogenetic results, strain LSR1 is considered to represent a novel species of a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is LSR1 (=GDMCC 1.1549=JCM 33645).

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  • Accepted:
  • Published Online:
Keyword(s): microaerophilic , Parasulfuritortus cantonensis , sulfur-oxidizing bacterium and Thiobacillaceae
Funding
This study was supported by the:
  • Fundamental Research Funds for the Central Universities (Award 2019ZD33)
    • Principle Award Recipient: JianfeiLuo
  • National Natural Science Foundation of China (Award 41977034)
    • Principle Award Recipient: WeitieLin
  • National Natural Science Foundation of China (Award 91951118)
    • Principle Award Recipient: JianfeiLuo
© 2021 The Authors
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2021-01-27
2024-05-06
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Parasulfuritortus cantonensis gen. nov., sp. nov., a microaerophilic sulfur-oxidizing bacterium isolated from freshwater sediment
Int J Syst Evol Microbiol 71, 004657 (2021); https://doi.org/10.1099/ijsem.0.004657
/content/journal/ijsem/10.1099/ijsem.0.004657
/content/journal/ijsem/10.1099/ijsem.0.004657
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