1887

Abstract

Cockle mortality events have been reported in northern France since 2012. In the present study, we describe and investigate the implication of a potential bacterial causative agent in cockle mortality. Bacteria isolated from five different cockle mortality events were characterized and studied. Using phenotypic analysis combined with DNA–DNA hybridization (DDH) and whole genome sequencing, the isolates were shown to belong to , a species regularly detected in France during oyster mortality events. Comparison of the strains from cockles with strains from French oysters and the type strain showed that the strains from cockles were genetically different to those from oysters and also different to the type strain. Moreover, the cockle and oyster strains were classified into two different, but close, groups both separated from the type strain by: (1) analyses of the gene sequences; (2) DDH assays between 12/122 3T3 (LMG 31436=DSM 109723), a representative cockle strain, 02/041 (CIP 109791=LMG 24517) representative oyster strain and type strain LMG 7909; (3) average nucleotide identity values calculated on the genomes; and (4) phenotypic traits. Finally, results of MALDI-TOF analyses also revealed specific peaks discriminating the three representative strains. The toxicity of representative strains of these cockle isolates was demonstrated by experimental infection of hatchery-produced cockles. The data therefore allow us to propose two novel subspecies of : subsp. subsp. nov. for the cockle strains and subsp. subsp. nov. for the Pacific oyster strains, in addition to an emended description of the species .

Funding
This study was supported by the:
  • CélineGarcia , EU-funded project VIVALDI , (Award 678589)
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2021-01-28
2021-03-02
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