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Abstract

A Gram-stain-variable, aerobic, orange pigmented, catalase-positive and oxidase-negative, cocci-shaped bacterium, designated SM7_A14, isolated from glacier fed sediment sample collected from the Queen Maud Land, near India’s Maitri station in Antarctica. Phylogenetic analysis based on 16S rRNA gene sequences revealed highest sequence similarity with DSM 22350 (97.3 %), demonstrated distinct phylogenetic positioning of strain SM7_A14 within the genus . Growth of strain SM7_A14 occurs at 5–25 °C (optimum, 20 °C), pH 7.0–10 (optimum, pH 8.0) with 0–5 % NaCl (optimum 1–4 %, w/v). C anteiso, C anteiso, C iso and C anteiso A are the major fatty acids (>5 % of the total fatty acids). The polar lipid profile consisted of diphosphatidylglycerol and phosphatidylglycerol. The average nucleotide identity (ANI) and digital DNA–DNA hybridization values between SM7_A14 and DSM 22350 were 80.3 and 21.3 %, respectively. The genomic DNA G+C content of the strain SM7_A14 was 68.5 %. Distinguishing characteristics based on the polyphasic analysis indicates strain SM7_A14 as a novel species of genus for which the name sp. nov., is proposed. The type strain is SM7_A14 (=MCC 4327=JCM 33936=LMG 31795).

Funding
This study was supported by the:
  • NotApplicable , Department of Biotechnology, Ministry of Science and Technology , (Award BT/Coord.II/01/03/2016)
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