1887

Abstract

A Gram-stain-variable, aerobic, orange pigmented, catalase-positive and oxidase-negative, cocci-shaped bacterium, designated SM7_A14, isolated from glacier fed sediment sample collected from the Queen Maud Land, near India’s Maitri station in Antarctica. Phylogenetic analysis based on 16S rRNA gene sequences revealed highest sequence similarity with DSM 22350 (97.3 %), demonstrated distinct phylogenetic positioning of strain SM7_A14 within the genus . Growth of strain SM7_A14 occurs at 5–25 °C (optimum, 20 °C), pH 7.0–10 (optimum, pH 8.0) with 0–5 % NaCl (optimum 1–4 %, w/v). C anteiso, C anteiso, C iso and C anteiso A are the major fatty acids (>5 % of the total fatty acids). The polar lipid profile consisted of diphosphatidylglycerol and phosphatidylglycerol. The average nucleotide identity (ANI) and digital DNA–DNA hybridization values between SM7_A14 and DSM 22350 were 80.3 and 21.3 %, respectively. The genomic DNA G+C content of the strain SM7_A14 was 68.5 %. Distinguishing characteristics based on the polyphasic analysis indicates strain SM7_A14 as a novel species of genus for which the name sp. nov., is proposed. The type strain is SM7_A14 (=MCC 4327=JCM 33936=LMG 31795).

Funding
This study was supported by the:
  • Department of Biotechnology, Ministry of Science and Technology (Award BT/Coord.II/01/03/2016)
    • Principle Award Recipient: NotApplicable
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004641
2021-01-13
2021-10-18
Loading full text...

Full text loading...

References

  1. Parte AC. LPSN-list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 2014; 42:D613–D616 [View Article][PubMed]
    [Google Scholar]
  2. Pitt A, Schmidt J, Koll U, Hahn MW. Rhodoluna limnophila sp. nov., a bacterium with 1.4 Mbp genome size isolated from freshwater habitats located in Salzburg, Austria. Int J Syst Evol Microbiol 2019; 69:3946–3954 [View Article][PubMed]
    [Google Scholar]
  3. Park YH, Suzuki K, Yim DG, Lee KC, Kim E et al. Suprageneric classification of peptidoglycan group B actinomycetes by nucleotide sequencing of 5S ribosomal RNA. Antonie van Leeuwenhoek 1993; 64:307–313 [View Article][PubMed]
    [Google Scholar]
  4. Stackebrandt E, Rainey FA, Ward-Rainey NL. Proposal for a new Hierarchic classification system, actinobacteria classis nov. Int J Syst Bacteriol 1997; 47:479–491 [View Article]
    [Google Scholar]
  5. Suzuki K, Sasaki J, Uramoto M, Nakase T, Komagata K. Cryobacterium psychrophilum gen. nov., sp. nov., nom. rev., comb. nov., an obligately psychrophilic actinomycete to accommodate "Curtobacterium psychrophilum" Inoue and Komagata 1976. Int J Syst Bacteriol 1997; 47:474–478 [View Article][PubMed]
    [Google Scholar]
  6. Mei L, Piao Z, Hu J, Shi L, Bai Y et al. Lysinimonas yzui sp. nov., isolated from cattail root soil from mine tailings. Int J Syst Evol Microbiol 2020; 70:2003–2007 [View Article][PubMed]
    [Google Scholar]
  7. Evtushenko LI. Microbacteriaceae. In Whitman WB. editor Bergey’s Manual of Systematics of Archaea and Bacteria Hoboken, New Jersey: Wiley; 2015 p 807 ff
    [Google Scholar]
  8. Li H-R, Yu Y, Luo W, Zeng Y-X. Marisediminicola antarctica gen. nov., sp. nov., an actinobacterium isolated from the Antarctic. Int J Syst Evol Microbiol 2010; 60:2535–2539 [View Article][PubMed]
    [Google Scholar]
  9. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461–466 [View Article][PubMed]
    [Google Scholar]
  10. Sharma A, Shouche Y. Microbial Culture Collection (MCC) and international Depositary authority (IDA) at national centre for cell science, Pune. Indian J Microbiol 2014; 54:129–133 [View Article][PubMed]
    [Google Scholar]
  11. Sharma A, Dhar SK, Prakash O, Vemuluri VR, Thite V et al. Description of Domibacillus indicus sp. nov., isolated from ocean sediments and emended description of the genus Domibacillus. Int J Syst Evol Microbiol 2014; 64:3010–3015 [View Article][PubMed]
    [Google Scholar]
  12. Kajale S, Deshpande N, Pali S, Shouche Y, Sharma A. Natrialba swarupiae sp. nov., a halophilic archaeon isolated from a hypersaline lake in India. Int J Syst Evol Microbiol 2020
    [Google Scholar]
  13. Yoon SH, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically United database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article]
    [Google Scholar]
  14. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425
    [Google Scholar]
  15. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article]
    [Google Scholar]
  16. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article]
    [Google Scholar]
  17. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article]
    [Google Scholar]
  18. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
    [Google Scholar]
  19. Jani K, Feng GD, Zhu HH, Prakash O, Bandal J et al. Chakrabartia godavariana gen. nov., sp. nov., a novel member of the family Sphingomonadaceae isolated from the Godavari River, India. Int J Syst Evol Microbiol 2019; 69:2452–2458 [View Article]
    [Google Scholar]
  20. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012; 19:455–477 [View Article]
    [Google Scholar]
  21. Bosi E, Donati B, Galardini M, Brunetti S, Sagot M-F et al. MeDuSa: a multi-draft based scaffolder. Bioinformatics 2015; 31:2443–2451 [View Article]
    [Google Scholar]
  22. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article]
    [Google Scholar]
  23. Wattam AR, Davis JJ, Assaf R, Boisvert S, Brettin T et al. Improvements to PATRIC, the all-bacterial bioinformatics database and analysis resource center. Nucleic Acids Res 2017; 45:D535–D542 [View Article]
    [Google Scholar]
  24. Sharma A, Jani K, Feng GD, Karodi P, Vemuluri VR et al. Subsaxibacter sediminis sp. nov., isolated from Arctic glacial sediment and emended description of the genus Subsaxibacter. Int J Syst Evol Microbiol 2018; 68:1678–1682 [View Article]
    [Google Scholar]
  25. Jani K, Khare K, Senik S, Karodi P, Vemuluri VR et al. Corynebacterium godavarianum sp. nov., isolated from the Godavari river, India. Int J Syst Evol Microbiol 2018; 68:241–247 [View Article]
    [Google Scholar]
  26. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  27. Li AH, Liu HC, Xin YH, Kim SG, Zhou YG. Glaciihabitans tibetensis gen. nov., sp. nov., a psychrotolerant bacterium of the family Microbacteriaceae, isolated from glacier ice water. Int J Syst Evol Microbiol 2014; 64:579–587
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004641
Loading
/content/journal/ijsem/10.1099/ijsem.0.004641
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error