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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 2

sp. nov., isolated from fermented tea leaves in Thailand Free

Abstract

A Gram-stain-positive, catalase-negative, rod-shaped, non-motile, non-spore-forming, and facultatively anaerobic strain CRM56-3, isolated from fermented tea leaves collected from Chiang Rai province, Thailand, was characterized based on a polyphasic approach. The strain produced -lactic acid heterofermentatively from glucose. It grew at 15–42 °C (optimum at 30 °C), pH 3.5–8.0 (optimum pH 6.0) and in 1–4 % (w/v) NaCl. Strain CRM56-3 contained C, C cyclo 8, and C 7, and/or C 6c as major cellular fatty acids. Based on 16S rRNA gene sequence analysis, strain CRM56-3 belongs to the genus and was closely related to DSM 19909 (99.2 %), JCM 1123 (98.9 %), and DSM 15502 (98.7 %). The draft genome of strain CRM56-3 contained 2681617 bp with 2413 coding sequences and DNA G+C content determined from genome sequence of 44.5 mol%. The digital DNA–DNA hybridization (dDDH) between strain CRM56-3 and DSM 19909, JCM 1123, and DSM 15502 were 19.5, 20.4, and 21.6 %, respectively. The average nucleotide identity (ANIm) and the average amino acid identity (AAI) between strain CRM56-3 and closely related strains were lower than 85.0 and 80.0 %, respectively. The strain CRM56-3 was clearly distinguished from related species by its phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequence similarity, and the draft genome analysis. Therefore, the strain represents a novel species of the genus for which the name of sp. nov. is proposed. The type strain is CRM56-3 (=JCM 34223=LMG 31663=TISTR 2851).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): fermented tea leaves , lactic acid bacteria , polyphasic and Secundilactobacillus folii
Funding
This study was supported by the:
  • Ratchadapisek Somphot Fund, Chulalongkorn University for a Postdoctoral Fellowship
    • Principle Award Recipient: SukanyaPhuengjayaem
  • Grant for International Research Integration: Research Pyramid, Ratchadaphiseksomphot Endowment Fund (Award GCURP_58_01_33_01)
    • Principle Award Recipient: SomboonTanasupawat
© 2021 The Authors
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2021-01-22
2024-04-26
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Secundilactobacillus folii sp. nov., isolated from fermented tea leaves in Thailand
Int J Syst Evol Microbiol 71, 004635 (2021); https://doi.org/10.1099/ijsem.0.004635
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