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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 71, Issue 2

sp. nov., a heavy metal resistant bacterium isolated from coral Free

Abstract

A Gram-stain-negative, aerobic, mesophilic, non-motile bacterium, designated M0104, was isolated from a gorgonian coral collected from Xieyang island, Guangxi Province, PR China. Colonies of the strain were non-motile cocci and pink. The strain grew at 15–34 °C (optimum, 28 °C), pH 4.5–8.0 (optimum, pH 7.0) and with 0–4% (w/v) NaCl (optimum, 0–2 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain M0104 was closely related to JCM 31275 (96.2 %), KCTC 62043 (96.0 %), JCM 31878 (95.9 %) and KCTC 42542 (95.7 %). The strain had an assembly size of 5.0 Mb and a G+C content of 71.0mol%. Genes involved in copper, cadmium, lead, arsenic and zinc resistance were identified in the genome of strain M0104. The digital DNA–DNA hybridization and average nucleotide identity values between the genome sequence of strain M0104 and those of closely related type strains were 19.4–24.9 % and 74.3–81.8 %, respectively. Strain M0104 contained C 7, C 3, anteiso C and C as the major fatty acids (>7 %) and ubiquinone Q-10 as the sole isoprenoid quinone. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine were its major polar lipids. Based on its phenotypic, phylogenetic and chemotaxonomic properties, strain M0104 is proposed to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is M0104 (=KCTC 62359=MCCC 1K03632).

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  • Accepted:
  • Published Online:
Keyword(s): coral , heavy-metal resistance , Roseomonas and Roseomonas coralli sp. nov.
Funding
This study was supported by the:
  • The Special Project for the Base of Guangxi Science and Technology and Talents (Award AD17129019)
    • Principle Award Recipient: FajunJiang
  • Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry (Award 17-259-74)
    • Principle Award Recipient: FeiLi
  • Fundamental Research Funds for Guangxi Academy of Sciences (Award 2018YBJ303)
    • Principle Award Recipient: WenjinHu
  • Fundamental Research Funds for Guangxi Academy of Sciences (Award 2019YBJ101)
    • Principle Award Recipient: XinliPan
  • National Natural Science Foundation of Guangxi (Award 2014GXNSFDA118012)
    • Principle Award Recipient: FeiLi
  • the National Natural Science Foundation of China (Award 31560017))
    • Principle Award Recipient: FeiLi
© 2021 The Authors
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2021-01-06
2025-01-26
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Roseomonas coralli sp. nov., a heavy metal resistant bacterium isolated from coral
Int J Syst Evol Microbiol 71, 004624 (2021); https://doi.org/10.1099/ijsem.0.004624
/content/journal/ijsem/10.1099/ijsem.0.004624
/content/journal/ijsem/10.1099/ijsem.0.004624
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