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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 2

sp. nov., a phosphate-solubilizing bacterium isolated from a solar saltern Free

Abstract

A Gram-stain-negative, motile, rod-shaped, non-endospore-forming, aerobic and halophilic bacterium, designated strain YCWA18, was isolated from the sediment of Jimo-Daqiao saltern in China. This strain was able to grow at NaCl concentrations in the range 0.5–20 % (w/v) with optimum growth at 6 % (w/v) NaCl. Growth occurred at temperatures of 4–40 °C (optimum 28 °C) and pH 4.0–9.0 (optimum 7.0). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YCWA18 belonged to the genus and shared the highest sequence similarity of 98.7 % with DSM 23229. Moreover, the phylogenetic analysis based on the 23S rRNA gene sequence also confirmed the phylogenetic position of this novel strain. The predominant fatty acids were C, C cyclo and C 3-OH. The major isoprenoid quinone was Q-9 (94.2 %) and the polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), an unidentified aminolipid (AL), an unidentified phospholipids (PL) and two unidentified lipids (L). The complete genome of strain YCWA18 consisted of a single, circular chromosome of 3 624 619 bp, with an average G+C content of 59.1 mol%. A genome-based phylogenetic tree constructed using an up-to-date bacterial core gene set (UBCG) showed that strain YCWA18 formed a clade with DSM 23229. However, the level of the ANI and dDDH values between strain YCWA18 and DSM 23229 were 82.3 and 24.6 %, respectively, which were low enough to distinguish strain YCWA18 from DSM 23229. Overall, based on the phenotypic, chemotaxonomic, phylogenetic and genomic analyses, strain YCWA18 represents a novel species of genus . The name sp. nov. is proposed, with the type strain YCWA18 (=CGMCC 1.9149=NCCB 100306).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Kushneria phosphatilytica , phosphate-solubilizing and taxonomy
Funding
This study was supported by the:
  • the Youth Talent Program Supported by Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Award 2018-MFS-T16, 2016LMFS-B08)
    • Principle Award Recipient: PingGao
  • the National Key R&D Program of China (Award 2017YFC1404500)
    • Principle Award Recipient: lingyunQu
© 2021 The Authors
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2020-12-23
2024-05-10
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Kushneria phosphatilytica sp. nov., a phosphate-solubilizing bacterium isolated from a solar saltern
Int J Syst Evol Microbiol 71, 004619 (2021); https://doi.org/10.1099/ijsem.0.004619
/content/journal/ijsem/10.1099/ijsem.0.004619
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