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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 71, Issue 1

gen. nov., sp. nov., a novel haloalkaliphile isolated from a laboratory culture cyanobacterium species and proposals of fam. nov., fam. nov., ord. nov. and ord. nov. Free

Abstract

A prosthecate bacterial strain, designated G-192, was isolated from decaying biomass of a haloalkaliphilic cyanobacterium sp. Z-T0701. The cells were aerobic, Gram-negative, non-endospore-forming and dimorphic, occurring either as sessile bacteria with a characteristic stalk or as motile flagellated cells. The strain utilized a limited range of substrates, mostly peptonaceous, but was able to degrade whole proteins. Growth occurred at 5–46 °C (optimum, 35–40 °C), pH 7.3–10.3 (optimum, pH 8.0–9.0), 0–14 % NaCl (v/w; optimum, 2.0–6.0 %, v/w). The G+C content of the genomic DNA of strain G-192 was 66.8%. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain G-192 formed a distinct evolutionary lineage within the family . Strain G-192 showed the highest 16S rRNA sequence similarity to ZYF765 (95.2%), GISW-4 (94.2%) and WD6-1 (95.5%). The major cellular fatty acids (>5% of the total) were C 9, C and 11-methyl-C ω7. The major polar lipids were glycolipids and phospholipids. The only respiratory quinone was ubiquinone-10 (Q-10). Based on polyphasic results including phylogenomic data, the novel strain could be distinguished from other genera, which suggests that strain G-192 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain is G-192 (=VKM B-3306=KCTC 72746). The strain is the first representative of the stalked bacteria associated with a haloalkaliphilic cyanobacterium. Based on phylogenomic indices and phenotypic data, it is proposed to evolve two novel families fam. nov. and fam. nov. out of the current family . In addition, it is proposed to place the first two families in the novel order ord. nov. and novel order ord. nov. is proposed to accommodate the family .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Alkalicaulis , alkaliphiles , Alphaproteobacteria , Hyphomonadaceae and stalked bacteria
Funding
This study was supported by the:
  • Российский Фонд Фундаментальных Исследований (РФФИ) (Award 18-04-00236)
    • Principle Award Recipient: VadimKevbrin
© 2021 The Authors

Erratum

An erratum has been published for this content:
Corrigendum: gen. nov., sp. nov., a novel haloalkaliphile isolated from a laboratory culture cyanobacterium species and proposals of fam. nov., fam. nov., ord. nov. and ord. nov
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Alkalicaulis satelles gen. nov., sp. nov., a novel haloalkaliphile isolated from a laboratory culture cyanobacterium Geitlerinema species and proposals of Maricaulaceae fam. nov., Robiginitomaculaceae fam. nov., Maricaulales ord. nov. and Hyphomonadales ord. nov.
Int J Syst Evol Microbiol 71, 004614 (2020); https://doi.org/10.1099/ijsem.0.004614
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