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Volume 71, Issue 1

gen. nov., sp. nov., a novel marine bacterium in the family , isolated from a tidal flat sediment Free

Abstract

Two marine bacterial strains, designated S2-4-21 and MT2-5-19, were isolated from two tidal flat sediments of cordgrass and adjacent oyster culture field in Quanzhou bay, China, respectively. Both strains were Gram-staining-negative, rod-shaped, non-flagellated, non-motile, aerobic, had NaCl requirements, and contained carotenoid and flexirubin pigments. The 16S rRNA gene sequence similarity (99.8%), average nucleotide identity value (99.4%) and average amino acid identity (99.3%) between strain S2-4-21 and strain MT2-5-19 strongly supported that they belonged to a single species. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S2-4-21 and strain MT2-5-19 formed a monophyletic branch affiliated to the family , sharing similarities of 94.6% with CY01 and 7SM30, and of 94.1 and 92.8% with MEBiC 12267 and DSM 19858, respectively. Phylogenomic analysis based on the whole genome sequences supported that the two strains formed a distinct monophyletic clade within members, which was phylogenetically different from the clades of and . The major respiratory quinone was menaquinone MK-6. The major fatty acids (>10%) consisted of C iso, C, summed feature 9 (C iso 9/C 10-methyl) and C iso 3-OH. The polar lipid profiles of strain S2-4-21 and strain MT2-5-19 are identical, including phosphatidylethanolamine, four unidentified aminolipids, and four unidentified lipids. The genomic size was 4.9–5.0 Mb with genomic DNA G+C content of 41.5 mol%. Based on the above characteristics, strains S2-4-21 and MT2-5-19 represented a novel species of a novel genus in the family . Thus, gen. nov. sp. nov. is proposed with type strain S2-4-21 (=MCCC 1K03818=KCTC 72152), and another strain MT2-5-19 (=KCTC 72539=MCCC 1K03874).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Flavobacteriaceae , Pareuzebyella sediminis , Spartina alterniflora and tidal flat sediment
Funding
This study was supported by the:
  • Specialized Research Fund for the Doctoral Program of Higher Education of China (Award H19005)
    • Principle Award Recipient: ZhaobinHuang
  • National Infrastructure of Microbial Resources of China (Award NIMR 2020-9)
    • Principle Award Recipient: ZhaobinHuang
© 2021 The Authors
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2020-12-10
2024-05-13
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Pareuzebyella sediminis gen. nov., sp. nov., a novel marine bacterium in the family Flavobacteriaceae, isolated from a tidal flat sediment
Int J Syst Evol Microbiol 71, 004606 (2020); https://doi.org/10.1099/ijsem.0.004606
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