1887

Abstract

The polyphasic taxonomic approach was used to characterize a novel bacteria strain, designated SG-8, which was isolated from intestinal content of a Pacific white shrimp (). Cells were Gram-stain-negative, aerobic, non-gliding rods. Growth occurred at 10–45 °C (optimum, 20–30 °C), pH 5.0–10.0 (optimum, 6.0–7.0) and in 0–6.0 % (w/v) NaCl (optimum, 0–4.0 %). The 16S rRNA gene sequence of strain SG-8 showed the highest sequence similarity to KMU-14 (98.6 %). On phylogenetic trees, strain SG-8 formed a stable cluster with KMU-14, SJ-36, 119BY6-57 and S2-C. The average nucleotide identity and digital DNA–DNA hybridization values between strain SG-8 and the four reference type strains listed above were 83.3, 82.3, 83.5, 83.3% and 22.8, 22.7, 22.7, 22.9 %, respectively. The major fatty acids (>5 %) were iso-C, summed feature 9 (iso-C 9 and/or 10-methyl C), iso-C, summed feature 3 (C 6 and/or C 7), iso-C, iso-C 3OH and iso-C. Ubiquinone-8 (Q-8) was the only respiratory quinone. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content was 68.8 mol%. Based on the results of genomic, phylogenetic, phenotypic and chemotaxonomic analyses, strain SG-8 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SG-8 (=GDMCC 1.1817=KACC 21942).

Funding
This study was supported by the:
  • AnzhangLi , Science and Technology Planning Project of Guangdong Province (CN) , (Award 2019B030316010)
  • AnzhangLi , Pearl River S and T Nova Program of Guangzhou , (Award 201806010065)
  • HonghuiZhu , GDAS' Special Project of Science and Technology Development , (Award 2020GDASYL-20200302002)
  • AnzhangLi , National Natural Science Foundation of China , (Award 32070115)
  • AnzhangLi , Key-Area Research and Development Program of Guangdong Province , (Award 2018B020205002)
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2021-02-23
2021-02-27
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