1887

Abstract

Six phytopathogenic bacterial strains, MAFF 301512, MAFF 301513, MAFF 301514, MAFF 301515, MAFF 301516 and MAFF 301517, were isolated from soft rot lesions of onion ( L.) in Japan. The cells were Gram-reaction-negative, aerobic, non-spore-forming, motile with one or two polar flagella and rod-shaped. Analysis of their 16S rRNA gene sequences showed that they belong to the genus , with the highest similarities to DSM 14936 (99.86 %), OLi (99.85 %), DSM 14937 (99.79 %) and KMM 3447 (99.79 %). Their genomic DNA G+C content was 60.9 mol% and the major fatty acids (>5 % of the total fatty acids) present were C, summed feature 3 (C ω7/C ω6), summed feature 8 (C ω7 /C ω6) and C cyclo. Phylogenetic and phylogenomic analyses based on the gene and whole genome sequences, respectively, demonstrated that the strains belong to the subgroup, but form a monophyletic and robust clade, with as their neighbour. Between the strains and , the average nucleotide identity scores were 95.63–95.70 %, whereas the digital DNA–DNA hybridization scores of the strains against their closest relatives, including , were 65.4 % or less, which are lower than the 70 % cut-off for prokaryotic species delineation. The strains were differentiated from their closest relatives by phenotypic characteristics, pathogenicity in onion and cellular fatty acid composition. The phenotypic, chemotaxonomic and genotypic data showed that the strains represent a novel species, proposed to be named sp. nov., with MAFF 301514 (=ICMP 23680) being the type strain.

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2020-12-03
2021-11-27
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