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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 3

sp. nov., an endophytic bacterium isolated from roots Free

Abstract

A Gram-stain-positive, facultatively anaerobic, rod-shaped, endospore-forming, oxidase-positive, and catalase-negative strain designated as BRMEA1 was isolated from the surface-sterilized roots. Growth of strain BRMEA1 was found to occur at pH 6.0–8.0 (optimum, pH 7.0), 15–50 °C (optimum, 25–30 °C) and in the absence of NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BRMEA1 formed a lineage within the genus (family ) and showed the highest sequence similarity to DSM 15600 (98.3 %) and KCTC 13885 (98.2 %), and less than 98.2 % 16S rRNA gene sequence similarity to the other members of the genus . Whole-genome analysis of strain BRMEA1 comprised a circular chromosome (5 632 809 bp in size) with 38.5 mol% G+C content. Digital DNA–DNA hybridization analyses revealed that strain BRMEA1 showed 20.5 and 22.0% genomic DNA relatedness with the closest species DSM 15600 and KCTC 13885, respectively. The whole-genome sequence of strain BRMEA1 showed the presence of 11 specific conserved signature indels for the genus . The major cellular fatty acids (>10 %) of strain BRMEA1 were found to be iso-C and anteiso-C, while the major polar lipids were found to be diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Polyphasic analysis results revealed that BRMEA1 represents a novel species of the genus , with the proposed name sp. nov. The type strain is BRMEA1 (=KCTC 43208=CCTCC AB 2020071).

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Keyword(s): endophytic bacterium , Neobacillus endophyticus , novel species , phylogeny and polyphasic taxonomy
Funding
This study was supported by the:
  • Korea Research Institute of Bioscience and Biotechnology (Award KGM5282021)
    • Principle Award Recipient: ChaYoung Kim
  • National Research Foundation of Korea (Award RBM0142011)
    • Principle Award Recipient: JiyoungLee
© 2021 The Authors
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2021-02-23
2024-05-11
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Neobacillus endophyticus sp. nov., an endophytic bacterium isolated from Selaginella involvens roots
Int J Syst Evol Microbiol 71, 004581 (2021); https://doi.org/10.1099/ijsem.0.004581
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