Full text loading...
Abstract
A group of four psychrotrophic bacterial strains was isolated on James Ross Island (Antarctica) in 2013. All isolates, originating from different soil samples, were collected from the ice-free northern part of the island. They were rod-shaped, Gram-stain-negative, and produced moderately slimy red-pink pigmented colonies on R2A agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, whole-genome sequencing, MALDI-TOF MS, rep-PCR analyses, chemotaxonomic methods and extensive biotyping was used to clarify the taxonomic position of these isolates. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates belonged to the genus Hymenobacter . The closest relative was Hymenobacter humicola CCM 8763T, exhibiting 98.3 and 98.9% 16S rRNA pairwise similarity with the reference isolates P5342T and P5252T, respectively. Average nucleotide identity, digital DNA–DNA hybridization and core gene distances calculated from the whole-genome sequencing data confirmed that P5252T and P5342T represent two distinct Hymenobacter species. The menaquinone systems of both strains contained MK-7 as the major respiratory quinone. The predominant polar lipids for both strains were phosphatidylethanolamine and one unidentified glycolipid. The major components in the cellular fatty acid composition were summed feature 3 (C16:1 ω7c/C16:1ω6c), C16:1ω5c, summed feature 4 (anteiso-C17:1 B/iso-C17:1 I), anteiso-C15:0 and iso-C15 : 0 for all isolates. Based on the obtained results, two novel species are proposed, for which the names Hymenobacter terrestris sp. nov. (type strain P5252T=CCM 8765T=LMG 31495T) and Hymenobacter lapidiphilus sp. nov. (type strain P5342T=CCM 8764T=LMG 30613T) are suggested.
- Received:
- Accepted:
- Published Online:
Funding
-
Ministerstvo Školství, Mládeže a Tělovýchovy
(Award CZ.02.1.01/0.0/0.0/16_013/0001761)
- Principle Award Recipient: Lenka Micenková
-
Ministerstvo Školství, Mládeže a Tělovýchovy
(Award LM2015051)
- Principle Award Recipient: Petra Vídeňská
-
Ministerstvo Školství, Mládeže a Tělovýchovy
(Award LM2018127)
- Principle Award Recipient: Ondrej Šedo
-
Masarykova Univerzita
(Award MUNI/A/0824/2019)
- Principle Award Recipient: Roman Pantůček
-
Ministerstvo Školství, Mládeže a Tělovýchovy
(Award LM2018121)
- Principle Award Recipient: Ivo Sedláček
-
Ministerstvo Školství, Mládeže a Tělovýchovy
(Award LM2015078)
- Principle Award Recipient: Ivo Sedláček