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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 12

sp. nov. isolated from the Hapcheon–Changnyeong barrage area in the Nakdong river Free

Abstract

The novel bacterial strain C33 was isolated from a freshwater sample collected from the Hapcheon–Changnyeong barrage. The Gram-negative, motile, yellow-pigmented strain C33 was characterized as a rod-shaped and strictly aerobic bacterium. A 16S-rRNA phylogenetic analysis revealed that this strain was most closely related to V2M44, X1-8, and ZLT-5 with 97.1, 97.0, and 95.0 % 16S-rRNA sequence similarities, respectively. The genomic DNA GC content of strain C33 was estimated at 65.0 mol%. The average nucleotide identity of strain C33 relative to V2M44 and ZLT-5 was found to be 77.0 and 75.6%, with average amino-acid identities of 69.9, and 66.7%, and the digital DNA–DNA hybridization values of 21.3 and 17.7 %, respectively. The cells grew at 19–37 °C and pH 6–9 with 0–0.5 % (w/v) NaCl (optimum: 28 °C, pH 6.5, and 0 % NaCl). The major component identified in the polyamine pattern was -homospermidine, and the main ubiquinone was Q-10. The predominant polar lipids characterized were diphophatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, and sphingoglycolipid. Iso-C, C anteiso, and summed feature 3 (C 6 and/or C 7) were found to be the primary cellular fatty acids in strain C33. Based on these genotypic and phenotypic characteristics, strain C33 was classified as a novel species of the genus ; and the name sp. nov. is proposed (=KACC 21511=JCM 33880).

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  • Accepted:
  • Published Online:
Keyword(s): Alphaproteobacteria , freshwater , sphingoglycolipid , Sphingomonas , taxonomy and ubiquinone Q10
Funding
This study was supported by the:
  • Korea University (Award K2006821)
    • Principle Award Recipient: Woojun Park
  • the National Institute of Biological Resources (Award NIBR202002108)
    • Principle Award Recipient: Woojun Park
© 2020 The Authors
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2020-10-13
2024-04-26
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Sphingomonas changnyeongensis sp. nov. isolated from the Hapcheon–Changnyeong barrage area in the Nakdong river
Int J Syst Evol Microbiol 70, 6091 (2020); https://doi.org/10.1099/ijsem.0.004503
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