1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 2

sp. nov., a yeast species isolated from forest habitats Free

Abstract

Five yeast strains isolated from forest habitats in Hungary and Germany were characterized phenotypically and by sequencing of the D1/D2 domain of the large subunit rRNA gene and the ITS1-5.8S-ITS2 (ITS) region of the rRNA gene. The strains have identical D1/D2 domain and ITS region sequences. By sequence comparisons, and were identified as the closest relatives among the currently recognized yeast species. The DNA sequences of the investigated strains differ by 1.2 % (six substitutions) in the D1/D2 domain and by 3.5 % (12 substitutions and eight indels) in the ITS region from the type strain of (CBS 8675) while by 1.2 % (six substitutions and one indel) in the D1/D2 domain and by 7 % (32 substitutions and seven indels) in the ITS region from the type strain of (CBS 5107). Because the intraspecies heterogeneity seems to be very low and the distance to the most closely related species is above the commonly expected level for intraspecies variability sp. nov. (holotype, CBS 16335; isotype, NCAIM Y.02233; MycoBank no., MB 835268) is proposed to accommodate the above-noted five yeast strains. Phenotypically the novel species can be distinguished from and by the formation of ascospores. forms one or two hat-shaped ascospores per ascus on many different media as well as well-developed pseudohyphae and true hyphae. Additionally, we propose the transfer of three anamorphic members of the sub-clade to the genus as the following new taxonomic combinations f.a., comb. nov., f.a., comb. nov. and f.a., comb. nov.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Cyberlindnera sylvatica , forest habitats and novel yeast species
Funding
This study was supported by the:
  • Education of the Hungarian Ministry of Human Capacities and by the European Union and co-financed by the European Social Fund (Award . EFOP-3.6.3-VEKOP-16-2017-00005)
    • Principle Award Recipient: Gábor Péter
  • Deutsche Bundesstiftung Umwelt (Award 34053/01-32)
    • Principle Award Recipient: Michael Brysch-Herzberg
© 2021 The Authors
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004477
2021-01-28
2024-05-01
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/71/2/ijsem004477.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.004477&mimeType=html&fmt=ahah

References

  1. Kurtzman CP, Robnett CJ, Basehoar-Powers E. Phylogenetic relationships among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyces gen. nov. FEMS Yeast Res 2008; 8:939–954 [View Article][PubMed]
     [Google Scholar]
  2. Minter D. Cyberlindnera, a replacement name for Lindnera Kurtzman, et al., nom. illegit. Mycotaxon 2009; 110:473–476
     [Google Scholar]
  3. Cadete RM, Cheab MA, Santos RO, Safar SV, Zilli JE et al. Cyberlindnera xylosilytica sp. nov., a xylitol-producing yeast species isolated from lignocellulosic materials. Int J Syst Evol Microbiol 2015; 65:2968–2974 [View Article][PubMed]
     [Google Scholar]
  4. Guamán-Burneo MC, Dussán KJ, Cadete RM, Cheab MA, Portero P et al. Xylitol production by yeasts isolated from rotting wood in the Galápagos Islands, Ecuador, and description of Cyberlindnera galapagoensis f.a., sp. nov. Antonie van Leeuwenhoek 2015; 108:919–931 [View Article][PubMed]
     [Google Scholar]
  5. Poomtien J, Jindamorakot S, Limtong S, Pinphanichakarn P, Thaniyavarn J. Two new anamorphic yeasts species, Cyberlindnera samutprakarnensis sp. nov. and Candida thasaenensis sp. nov., isolated from industrial wastes in Thailand. Antonie van Leeuwenhoek 2013; 103:229–238 [View Article][PubMed]
     [Google Scholar]
  6. Kurtzman CP. Lindnera Kurtzman, Robnett & Basehoar-Powers. Yeasts: A Taxonomic Study 1-3, 5th ed. 2011 pp 521–543
     [Google Scholar]
  7. Brysch-Herzberg M, Seidel M. Distribution patterns of Saccharomyces species in cultural landscapes of Germany. FEMS Yeast Res 2017; 17: [View Article][PubMed]
     [Google Scholar]
  8. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for Isolation, Phenotypic Characterization and Maintenance of Yeasts.yeasts: a Taxonomic Study 1-3, 5th ed. 2011 pp 87–110
     [Google Scholar]
  9. Brysch-Herzberg M, Seidel M. Yeast diversity on grapes in two German wine growing regions. Int J Food Microbiol 2015; 214:137–144 [View Article][PubMed]
     [Google Scholar]
  10. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998; 73:331–371 [View Article][PubMed]
     [Google Scholar]
  11. White T, Bruns T, Lee S, Taylor J. Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. PCR Protocols: a Guide to Methods and Applications London: Academic Press; 1990 pp 315–322
     [Google Scholar]
  12. Nei M, Kumar S. Molecular evolution and phylogenetics: Oxford university press; 2002
  13. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. mega X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35:1547–1549 [View Article][PubMed]
     [Google Scholar]
  14. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
     [Google Scholar]
  15. Nirenberg H. Untersuchungen über die morphologische und biologische Differenzierung in der Fusarium-Sektion Liseola. Mitteilungen aus der Biologischen Bundesanstalt für Land- und Forstwirtschaft, Berlin Dahlem; 1976; 1691–117
  16. Vu D, Groenewald M, Szöke S, Cardinali G, Eberhardt U et al. DNA barcoding analysis of more than 9 000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation. Stud Mycol 2016; 85:91–105 [View Article][PubMed]
     [Google Scholar]
  17. Lachance MA. CP Kurtzman’s evolving concepts of species, genus and higher categories. FEMS Yeast Res 2018; 18:foy103
     [Google Scholar]
  18. Daniel H-M, Vrancken G, Takrama JF, Camu N, De Vos P et al. Yeast diversity of Ghanaian cocoa bean heap fermentations. FEMS Yeast Res 2009; 9:774–783 [View Article][PubMed]
     [Google Scholar]
  19. Barnett JA, Payne RW, Yarrow D. Yeasts: Characteristics and Identification, 3rd ed. Cambridge, UK; New York, NY, USA: Cambridge University Press; 2000 p 1139
     [Google Scholar]
  20. Kurtzman C, Fell JW, Boekhout T. The yeasts: a taxonomic study. Elsevier 2011
     [Google Scholar]
  21. Kurtzman CP. Four new yeasts in the Pichia anomala clade. Int J Syst Evol Microbiol 2000; 50 Pt 1:395–404 [View Article][PubMed]
     [Google Scholar]
  22. Lachance MA, Boekhout T, Scorzetti G, Fell JW, Kurtzman CP. Candida Berkhout (1923). In Kurtzman C, Fell JW, Boekhout T. (editors) The yeasts: a Taxonomic Study Amsterdam: Elsevier; 2011 pp 987–1278
     [Google Scholar]
  23. Daniel H-M, Lachance M-A, Kurtzman CP. On the reclassification of species assigned to Candida and other anamorphic ascomycetous yeast genera based on phylogenetic circumscription. Antonie van Leeuwenhoek 2014; 106:67–84 [View Article][PubMed]
     [Google Scholar]
  24. Shen X-X, Opulente DA, Kominek J, Zhou X, Steenwyk JL et al. Tempo and mode of genome evolution in the budding yeast subphylum. Cell 2018; 175:e201533–1545 [View Article][PubMed]
     [Google Scholar]
  25. Jindamorakot S, Am-In S, Thuy TT, Duy ND, Kawasaki H et al. Candida easanensis sp. nov., Candida pattaniensis sp. nov. and Candida nakhonratchasimensis sp. nov., three new species of yeasts isolated from insect frass in Thailand. J Gen Appl Microbiol 2004; 50:261–269 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004477
Loading
Cyberlindnera sylvatica sp. nov., a yeast species isolated from forest habitats
Int J Syst Evol Microbiol 71, 004477 (2021); https://doi.org/10.1099/ijsem.0.004477
/content/journal/ijsem/10.1099/ijsem.0.004477
/content/journal/ijsem/10.1099/ijsem.0.004477
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error