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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 11

sp. nov., a novel member of isolated from activated sludge collected in Hong Kong Free

Abstract

A floc-forming bacterial strain, designated HKLI-1, was isolated from the activated sludge of a municipal sewage treatment plant in Hong Kong SAR, PR China. Cells of this strain were Gram-stain-negative, strictly aerobic, catalase- and oxidase-positive, rod-shaped and motile by means of a single polar flagellum. Growth occurred at 18–37 °C (optimum, 28 °C), pH 5.5–9.0 (optimum, pH 7.5) and with 0–8.0 % (w/v) NaCl (optimum, 1–1.5 %) concentration. The major fatty acids of strain HKLI-1 were C and summed feature 3 (C ω7 and/or C ω6). The polar lipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and three unidentified lipids. The DNA G+C content was 63.5 mol% from whole genomic sequence analysis. Based on the results of 16S rRNA gene sequences analysis, this strain should be assigned to the genus and is closely related to DQS-4 (94.93 % 16S rRNA gene sequence pairwise similarity), MF63 (94.91 %) and SWub3 (94.01 %), but separate from them by large distances in different phylogenetic trees. Based on whole genome analysis, the orthologous average nucleotide identity and DNA–DNA hybridization values against four of the closest relatives were 73.03–74.83 and 17.2–23.0 %, respectively. The phylogenetic, genotypic, phenotypic and chemotaxonomic data demonstrated that strain HKLI-1 could be distinguished from its phylogenetically related species, and that this strain represented a novel species within the genus , for which the name sp. nov. is proposed. The type strain is HKLI-1 (= 72659=CCTCC AB 2019312).

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Keyword(s): activated sludge , Azoarcus halotolerans , floc formation , novel species and salt tolerance
Funding
This study was supported by the:
  • the Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences (A) (Award XDA23040401)
    • Principle Award Recipient: Dongru Qiu
  • National Natural Science Foundation of China (Award no. 91751106)
    • Principle Award Recipient: Dongru Qiu
© 2020 The Authors
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2020-09-18
2024-05-04
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Azoarcus halotolerans sp. nov., a novel member of Rhodocyclaceae isolated from activated sludge collected in Hong Kong
Int J Syst Evol Microbiol 70, 5799 (2020); https://doi.org/10.1099/ijsem.0.004476
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