1887

Abstract

A novel actinobacterial strain (TRM 68085) was isolated from soil of wetland. A polyphasic approach was used to study the taxonomy of TRM 68085 and the results showed a range of phylogenetic and chemotaxonomic properties consistent with those of the genus . Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TRM 68085 showed the highest similarity value to 1H-SSA4 (98.6 %), and phylogenetically clustered with ZX01(97.5 %) and NBRC 13050 (97.4 %). The genomic DNA G+C content of strain TRM 68085 based on the genome sequence was 71.4 mol%. The levels of DNA–DNA relatedness between the genome of the isolate and its nearest phylogenetic neighbours, 1H-SSA4, ZX01 and NBRC 13050, were 19.2±0.4, 21.8±0.5 and 19.3±0.6 %, respectively. Chemotaxonomic data revealed that strain TRM 68085 possessed MK-9(H) and MK-9(H) as the predominant menaquinones. -Diaminopimelic acid and a small amount of -diaminopimelic acid were the diagnostic diamino acids. Ribose, xylose, glucose and galactose were the whole-cell sugars. The major cellular fatty acids were C (25.4 %) and iso-C (18.3 %). On the basis of these genotypic and phenotypic data, it is concluded that strain TRM 68085 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TRM 68085 (=CCTCC AA2019031=LMG 31492).

Funding
This study was supported by the:
  • Tarim University graduate research and innovation projects (project no.TDGR1201808) (Award TDGR1201808)
    • Principle Award Recipient: Panpan Liu
  • National Natural Science Foundation of China (Award U1703236)
    • Principle Award Recipient: Li-Li Zhang
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2020-09-17
2022-09-25
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