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Volume 70, Issue 10

sp. nov., isolated from the gut of plastic-eating larvae of the coleopteran insect Free

Abstract

A bacterial strain, BIT-d1, was isolated from the gut of plastic-eating larvae of the coleopteran insect . Its taxonomic position was analysed using a polyphasic approach. Cells were white-pigmented, Gram-stain-negative, non-motile, long rods without flagella. The 16S rRNA gene sequence (1401 bp) of strain BIT-d1 showed highest similarity (98.0%) to SM1 and 96.6~92.6 % similarity to the other species of the genus . The results of phylogenetic analyses, based on the 16S rRNA gene, concatenated sequences of six housekeeping genes (, , , , and ) and genome sequences, placed strain BIT-d1 in a separate lineage among the genus , family . The major isoprenoid quinone was menaquinone-6 (MK-6) and the major fatty acids were C iso, C iso 3-OH and summed feature 9 (comprising iso-C  9 and/or C 10-methyl), which were similar to other members in the genus DNA–DNA hybridization and average nucleotide identity calculations plus physiological and biochemical tests exhibited the genotypic and phenotypic differentiation of strain BIT-d1 from the other members of the genus . Therefore, strain BIT-d1 is considered to represent a novel species within the genus , for which the name sp. nov is proposed. The type strain is BIT-d1 (=CGMCC 1.17043=KCTC 72447).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): gut bacteria , Myroides , plastic biodegradation and polystyrene
Funding
This study was supported by the:
  • Beijing Institute of Technology Research Fund Program for Young Scholars (Award 3160011181804)
    • Principle Award Recipient: Yu Yang
  • China Association of Science and Technology (Award 2017QNRC001)
    • Principle Award Recipient: Yu Yang
  • National Natural Science Foundation of China (Award 51603004)
    • Principle Award Recipient: Yu Yang
  • National Natural Science Foundation of China (Award 31961133015)
    • Principle Award Recipient: Yu Yang
© 2020 The Authors
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2020-09-07
2024-05-14
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Myroides albus sp. nov., isolated from the gut of plastic-eating larvae of the coleopteran insect Zophobas atratus
Int J Syst Evol Microbiol 70, 5460 (2020); https://doi.org/10.1099/ijsem.0.004429
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