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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 10

sp. nov., isolated from Gahai Saline Lake, PR China Free

Abstract

A Gram-stain-negative, aerobic, non-motile and ovoid- to rod-shaped bacterial strain, designated GH877, was isolated from a water sample of Gahai saline lake in Qaidam Basin,PR China. The isolate grew at 5–45 °C, pH 6.0–9.0 (optima, 37 °C and pH 7.5) and with 0.5–20 % (w/v) NaCl (optimum, 2.0 %). The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain GH877 belonged to the genus , and had highest 16S rRNA gene sequence similarity of 97.7 % to 81-2, followed by HJ50 (97.5 %) and GSW-M15 (96.8 %). Genome sequencing revealed a genome size of 3 378 519 bp and a G+C content of 59.8 mol %. Up-to-date bacterial core gene set analysis indicated that strain GH877 represents one independent lineage with DSM29589. The average nucleotide identity values of GH877 with 81-2 and HJ50 are 80.7 and 77.3 %, respectively. DNA–DNA hybridization values between strain GH877 and 81-2 and HJ50 are 23.2 and 20.0 %, respectively. Q-10 was the predominant respiratory quinone and summed feature 8 (C ω7 and/or Cω6) and C were the major cellular fatty acids. The polar lipids of strain GH877 consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and two unidentified phosphoglycolipids. Based on polyphasic taxonomic analysis, strain GH877 is proposed to represent a novel species of the genus , for which the name sp. nov. is proposed. (type strain GH877=CGMCC 1.13971=KCTC 72576).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): polyphasic taxonomy , Roseovarius gahaiensis and saline lake
Funding
This study was supported by the:
  • Hunan Province Science and Technology Major Project (Award 2018SK1010)
    • Principle Award Recipient: Xia Sun
  • National key research and development program (Award 2018YFC0408103)
    • Principle Award Recipient: Fang Wang
© 2020 The Authors
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2020-09-08
2025-01-16
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Roseovarius gahaiensis sp. nov., isolated from Gahai Saline Lake, PR China
Int J Syst Evol Microbiol 70, 5330 (2020); https://doi.org/10.1099/ijsem.0.004411
/content/journal/ijsem/10.1099/ijsem.0.004411
/content/journal/ijsem/10.1099/ijsem.0.004411
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