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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 9

sp. nov., isolated from the surface of coralline algae Free

Abstract

A Gram-stain-negative, oxidase- and catalase-positive, facultative anaerobic and rod-shaped bacterium, designated strain SM1977, was isolated from the surface of coralline algae collected from the intertidal zone at Qingdao, PR China. The strain grew at 10–35 °C, pH 4.5–8.5 and with 1–8.5% (w/v) NaCl. It reduced nitrate to nitrite and hydrolysed Tween 20 and DNA. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SM1977 was affiliated with the genus , having the highest sequence similarity (97.6 %) to the type strain of , followed by those of another five species (95.6–97.6 %) in the Rumoiensis clade of the genus . However, the DNA–DNA hybridization (75.3–75.9 %) and average nucleotide identity (21.6–22.8 %) values of SM1977 against these close relatives were all below the corresponding thresholds to discriminate bacterial species. The major fatty acids were summed feature 3 (C 7 and/or C 6), C and summed feature 8 (C 6 and /or C 7). The predominant polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The sole respiratory quinone was Q-8. The genomic DNA G+C content of strain SM1977, determined from the obtained whole genomic sequence, was 42.3 mol%. On the basis of the polyphasic results obtained in this study, strain SM1977 is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is SM1977 (=MCCC 1K04351=KCTC 72847).

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  • Accepted:
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Keyword(s): algae-attached bacteria , intertidal zone , polyphasic taxonomy , Rumoiensis clade and Vibrio algicola sp. nov.
Funding
This study was supported by the:
  • State Scholarship Fund issued by China Scholarship Council (Award 201906225012)
    • Principle Award Recipient: Xi-Ying Zhang
  • Science and Technology Basic Resources Investigation Program of China (Award 2017FY100804)
    • Principle Award Recipient: Xi-Ying Zhang
  • Taishan Scholars Program of Shandong Province (Award tspd20181203)
    • Principle Award Recipient: Yu-Zhong Zhang
  • AoShan Talents Cultivation Program Supported by Qingdao National Laboratory for Marine Science and Technology (Award 2017ASTCP-OS14)
    • Principle Award Recipient: Yu-Zhong Zhang
  • National Science Foundation of China (Award 31870052)
    • Principle Award Recipient: Xiu-Lan Chen
  • National Science Foundation of China (Award 31971535)
    • Principle Award Recipient: Xiao-Yan Song
  • National Science Foundation of China (Award U1706207)
    • Principle Award Recipient: Yu-Zhong Zhang
  • National Key Research and Development Program of China (Award 2018YFC1406700)
    • Principle Award Recipient: Yu-Zhong Zhang
  • National Key Research and Development Program of China (Award 2018YFC0310704)
    • Principle Award Recipient: Xiu-Lan Chen
© 2020 The Authors
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2020-08-19
2024-04-26
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Vibrio algicola sp. nov., isolated from the surface of coralline algae
Int J Syst Evol Microbiol 70, 5149 (2020); https://doi.org/10.1099/ijsem.0.004394
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